Limbal Stem Cells
Limbal Stem Cells - Related Videos
04:55
A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
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Cited by 11 •
2016
The following article provides an easy, reproducible technique to effectively create a sustainable mouse model of limbal stem cell deficiency (LSCD). This animal model is useful in testing and comparing the efficacy of treatments for limbal stem cell diseases.
11:13
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
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Cited by 27 •
2016
An example of adenoviral gene therapy in the human diabetic organ-cultured corneas is presented towards the normalization of delayed wound healing and markedly reduced epithelial stem cell marker expression in these corneas. It also describes the optimization of this process in stem cell-enriched limbal epithelial cultures.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
10:07
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
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Cited by 28 •
2018
This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.
10:11
Isolation and Identification of Limbal Niche Cells
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Cited by 3 •
2023
Here, we present a protocol to isolate and identify the human limbal niche cells.
FAQs
The niche coordinates two opposing requirements: preserving a self-renewing limbal stem cell population and producing transient amplifying progenitors for epithelial replacement. These progenitors proliferate, migrate across the corneal surface, and differentiate into mature epithelial cells. This organization allows the tissue to maintain its transparent epithelial covering while responding to ongoing repair demands.
Transient amplifying progenitors provide an intermediate stage between long-term stem cell maintenance and mature corneal epithelial cells. Their ability to proliferate expands the number of replacement cells before migration and differentiation occur. In bioengineering studies, this behavior is important because successful cultivated-cell approaches must support expansion without losing the capacity to contribute to epithelial repair.
Engineered substrates provide designed environments for cultivated limbal cells during expansion and tissue restoration. Together with biomaterial scaffolds, they can support cell growth and help organize cells for application to damaged ocular surfaces. Studying these environments also gives bioengineers a way to investigate how the stem cell niche contributes to regeneration and implant development.
A broad approach begins with cultivated limbal cells, followed by their expansion using suitable biomaterial scaffolds or engineered substrates. The supported cell population is then directed toward restoring a damaged ocular surface. This workflow connects cell cultivation with material design, allowing researchers to evaluate how engineered environments contribute to tissue regeneration and ocular repair.
These approaches are especially relevant when limbal stem cell deficiency results from injury or disease and the ocular surface requires restoration. Cultivated cells and supporting materials offer a framework for developing treatments aimed at damaged surfaces. Their use also extends beyond therapy, providing models for studying tissue-resident stem cells and regenerative processes.
Combining cells with biomaterial scaffolds or engineered substrates can reveal how material-supported environments affect cell expansion and ocular surface restoration. The resulting systems support research on stem cell niches, tissue regeneration, and ocular implants. In this context, bioengineering links cellular behavior with scaffold and substrate design rather than examining limbal cells in isolation.