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A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells
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Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research (Video) | JoVE
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Isolation and Identification of Limbal Niche Cells

Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research (Video) | JoVE

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

DOI:
10.3791/65618-v

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01:28 min

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October 27, 2023

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, , , , , , , , , ,
1Department of Ophthalmology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, 2Institute of Medicine Nursing,Hubei University of Medicine, 3Department of Ophthalmology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

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Keywords: Limbal Niche CellsLimbal Stem Cell DeficiencySingle Cell SequencingMesenchymal Stem CellsCollagenase ATrypsin-EDTAModified Embryonic Stem Cell MediumMatrigelImmunofluorescenceReal-time Quantitative PCRFlow CytometryStem Cell MarkersVIMCD90CD105PDGFR-betaPan-CKCD73SCF

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