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Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

DOI:
10.3791/54058-v

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11:13 min

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April 07, 2016

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, ,
1Eye Program, Board of Governors Regenerative Medicine Institute, Departments of Biomedical Sciences and Neurosurgery,Cedars-Sinai Medical Center, 2David Geffen School of Medicine,University of California, Los Angeles

Chapters

  • 00:05Title
  • 01:09Epithelial Wound Healing in Organ-cultured Corneas
  • 02:57Adenoiral Transduction of Organ-cultured Corneas
  • 04:31Isolation of Limbal Cells and Maintenance of Stem Cell Enriched Cultures
  • 06:28Adenoiral Transduction of Cultured Limbal Cells
  • 07:55Results: Effects of Gene Therapy on Wound Healing and Stem Cell Marker Expression
  • 10:25Conclusion

Summary

Automatic Translation

Automatic Translation

An example of adenoviral gene therapy in the human diabetic organ-cultured corneas is presented towards the normalization of delayed wound healing and markedly reduced epithelial stem cell marker expression in these corneas. It also describes the optimization of this process in stem cell-enriched limbal epithelial cultures.

Tags

Keywords: Adenoviral Gene TherapyDiabetic KeratopathyWound HealingStem Cell Marker ExpressionHuman Organ-cultured CorneasLimbal Epithelial CellsEpithelial Wound HealingCorneal Stem Cell FunctionCorneal DiseasesStem Cell DysfunctionEpithelial DebridementCorneal HealingApoptotic Stromal Keratocytes

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