Metabolic Perturbation
Metabolic Perturbation - Related Videos
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
07:07
Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
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Cited by 13 •
2013
Here we describe a protocol for optogenetic manipulation of motoneuronal activity while monitoring changes in motor output (muscle contraction) in semi-intact Drosophila larvae using lasers within a conventional confocal microscope. This technique enables researchers to achieve local perturbation of neural activity in a few neuromeres to elucidate the dynamics of motor circuits.
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
The intervention can target different control points, including nutrient availability, enzyme activity, gene expression, or pathway inputs. Changing one of these points helps researchers observe whether downstream metabolite levels, flux, energy use, or cellular behavior shifts. Comparing responses across targets can separate effects associated with pathway regulation from those associated with a particular input or control point.
Metabolite levels and metabolic flux provide complementary views of pathway response. A level change shows that metabolite abundance shifted, whereas a flux change indicates altered movement through the pathway. Energy use and cellular behavior add functional context. Using these measurements together helps connect a biochemical change to broader consequences in the cell.
Changing pathway inputs or regulatory components can expose a metabolic bottleneck, where pathway performance is constrained at a particular point. The resulting response also helps connect genotype with phenotype by showing how altered gene expression or enzyme activity is reflected in metabolites, flux, energy use, or cellular behavior. This makes regulation and adaptation experimentally observable.
Researchers begin by selecting the metabolic feature to alter, such as nutrient availability, enzyme activity, gene expression, or a pathway input. They then measure relevant outcomes, including metabolite levels, flux, energy use, or cellular behavior. Matching the chosen intervention with the appropriate readout helps determine which pathway responses and functional effects accompany the altered metabolic state.
In bioengineering, these experiments can guide the design of engineered microbes and cells that produce valuable compounds. By revealing pathway regulation and bottlenecks, perturbations indicate where changing inputs, enzyme activity, or gene expression may improve a production strategy. The same measurements can support bioprocess optimization by linking cellular metabolic responses with process-relevant performance.
Beyond production systems, metabolic perturbation provides a way to study disease-associated metabolism and cellular adaptation. Controlled changes can reveal how cells respond through altered metabolite levels, flux, energy use, or behavior. In a bioengineering context, this connects mechanistic pathway analysis with the broader question of how biological systems maintain or change function when their metabolic conditions are modified.