Microfluidic Chamber Assays
Microfluidic Chamber Assays - Related Videos
03:50
Microscopic Analysis of Bacterial Growth Under Stress Using Microfluidic Chambers
0 Views •
2025
Source: Cayron, J., et al. Multi-scale Analysis of Bacterial Growth Under Stress Treatments. J. Vis. Exp. (2019).This video demonstrates how to prepare and use a microfluidic plate for imaging bacterial cells under stress conditions. It outlines the process of introducing a bacterial culture and a stress-inducing antibiotic medium, followed by cell loading and microscopic setup. Time-lapse imaging in phase-contrast mode is then used to observe the development of elongated, filamentous cells...
27:58
Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
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Cited by 8 •
2007
We demonstrate fabrication of a simple microfluidic device that can be integrated with standard electrophysiology setups to expose microscale surfaces of a brain slice in a well controlled manner to different neurotransmitters.
10:25
Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
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Cited by 9 •
2016
Platelet transfusion and hemostasis was modeled using blood reconstitution and microfluidic flow chambers to investigate the function of blood banking platelets. The data demonstrate the consequences of platelet storage lesion on hemostasis, in vitro.
12:42
Human Neutrophil Flow Chamber Adhesion Assay
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Cited by 12 •
2014
A method of quantitating neutrophil adhesion is reported. This method creates a dynamic flow environment similar to that encountered in a blood vessel. It allows the investigation of neutrophil adhesion to either purified adhesion molecules (ligand) or endothelial cell substrate (HUVEC) in a context similar to the in vivo environment with sheer stress.
10:35
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
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Cited by 1 •
2007
FAQs
Chamber geometry determines where cells or other biological samples reside and how fluids contact them. The dimensions and arrangement of channels can regulate exposure to chemical gradients, flow, surfaces, and neighboring cells. This spatial control helps researchers separate physical placement from biological response, making it possible to examine transport, barrier behavior, and cell interactions under defined experimental conditions.
These driving forces move small fluid volumes through the device, but they provide different ways to establish and maintain flow. Pumps offer an active means of directing fluid, whereas pressure differences or capillary forces can move fluid through channel networks without the same pumping arrangement. Their use determines how samples experience fluid movement and chemical exposure during measurement.
Gradients create spatially varying chemical conditions, allowing biological samples to encounter different exposures within the same engineered environment. Controlled positioning also supports studies of how cells respond to nearby surfaces or other cells. Together, these features make it possible to investigate localized behavior and cell–cell communication rather than relying only on measurements from uniformly mixed samples.
A device is configured with chambers and channels that establish the desired sample location, fluid movement, and exposure conditions. Researchers then monitor the biological response through microscopy, which is compatible with these platforms and can capture changes during the experiment. Parallel device designs can support repeated or comparative measurements while using small reagent volumes.
They are useful when researchers need controlled studies of cell behavior, transport, barrier function, or communication between cells. The platforms also support disease modeling, drug testing, and development of engineered tissue systems. Their controlled environments and microscopy compatibility are especially valuable when biological responses must be measured dynamically rather than inferred from a single endpoint.
Parallel designs allow multiple chamber conditions or samples to be examined within an organized device format, supporting comparative experiments. Because the chambers handle tiny volumes, the assays can reduce reagent use while maintaining precise control over exposure and observation. These features improve experimental resolution and help bioengineers evaluate biological responses across conditions more efficiently.