Near Infrared Absorbance
Near Infrared Absorbance - Related Videos
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Gas Absorber
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2023
Source: Michael G. Benton and Kerry M. Dooley, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Gas absorbers are used to remove contaminants from gas streams. Multiple designs are used to accomplish this objective1. A packed bed column uses gas and liquid streams running counter to each other in a column packed with loose packing materials, such as ceramics, metals, and plastics, or structured packing1. The packed bed uses surface area created by the packing to...
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Simulation, Fabrication and Characterization of THz Metamaterial Absorbers
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This protocol outlines the simulation, fabrication and characterization of THz metamaterial absorbers. Such absorbers, when coupled with an appropriate sensor, have applications in THz imaging and spectroscopy.
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
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Template Directed Synthesis of Plasmonic Gold Nanotubes with Tunable IR Absorbance
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Cited by 1 •
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Solution-suspendable gold nanotubes with controlled dimensions can be synthesized by electrochemical deposition in porous anodic aluminum oxide (AAO) membranes using a hydrophobic polymer core. Gold nanotubes and nanotube arrays hold promise for applications in plasmonic biosensing, surface-enhanced Raman spectroscopy, photo-thermal heating, ionic and molecular transport, microfluidics, catalysis and electrochemical sensing.
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Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
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Cited by 1 •
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Optical clearing techniques, such as absorbing molecules solutions, reduce light scattering temporarily to enhance biological imaging and offer biocompatible, reversible in vivo imaging strategies for deeply embedded organs. This protocol presents detailed experimental procedures as well as advanced image processing to achieve high-quality, dynamic imaging for real-time dynamics in living animals.
FAQs
Molecular bonds produce near infrared features through overtone and combination vibrations, which are higher-order or interacting vibrational responses. Because different bonds respond at particular wavelengths, the resulting pattern can contain chemical information about a sample. Interpreting that pattern also requires attention to concentration and optical path length, which influence the measured signal.
Concentration and optical path length influence how much near infrared light is attenuated before reaching the detector. Changes in either variable can alter spectral intensity even when the underlying material is otherwise similar. For this reason, comparisons between samples require controlled or documented measurement conditions, while calibration models can help relate spectral changes to biochemical conditions.
Scattering changes the recorded spectrum in addition to wavelength-specific molecular absorption. This effect is especially relevant when analyzing biological tissues or structured biomaterials, where internal structure can influence light propagation. Considering scattering helps separate chemical information from structural contributions, improving interpretation of spectra used to characterize composition or monitor changes in biological materials.
Spectroscopic instrumentation records the sample’s wavelength-dependent response, and calibration models connect those spectral patterns with measured chemical or biochemical conditions. Once established, the combined system can support rapid analysis of changing samples without requiring every result to be obtained through a separate direct assay. Model performance depends on representative calibration data and consistent measurement conditions.
Near infrared absorbance is useful when researchers need rapid information from biological tissues, fluids, or biomaterials while minimizing sample alteration. Its ability to capture chemical and structural information supports noninvasive monitoring and material characterization. In bioengineering studies, the approach can therefore follow changing biochemical conditions or compare biomaterials during development and evaluation.
In bioengineering, this approach can contribute to diagnostics, tissue engineering, biomedical device development, and process characterization. Measurements can help monitor biochemical changes, assess biomaterial behavior, or provide information relevant to biological systems and engineered constructs. Its value lies in combining spectroscopic data with calibration models to obtain rapid insights that support research decisions and process monitoring.