Shuttle Vector
Shuttle Vector - Related Videos
09:00
Cargo Loading onto Kinesin Powered Molecular Shuttles
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Cited by 4 •
2010
Molecular shuttles consisting of functionalized microtubules gliding on surface-adhered kinesin motor proteins can serve as a nanoscale transport system. Here, the assembly of a typical shuttle system is described.
07:18
An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
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Cited by 1 •
2022
Electroporation is a rapid, broadly adopted method for introducing exogenous DNA into the genus Rickettsia. This protocol provides a useful electroporation method for the transformation of obligate intracellular bacteria in the genus Rickettsia.
13:36
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
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Cited by 1 •
2015
Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.
09:20
Vectors in Multiple Directions
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2023
Source: Nicholas Timmons, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA This experiment demonstrates how vectors add and subtract in multiple directions. The goal will be to analytically calculate the addition or subtraction of multiple vectors and then to experimentally confirm the calculations. A vector is an object with both magnitude and direction. The magnitude of a vector is simply denoted as the length,...
09:16
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
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Cited by 13 •
2016
Vaccinia virus (VV) has been widely used in biomedical research and the improvement of human health. This article describes a simple, highly efficient method to edit the VV genome using a CRISPR-Cas9 system.
FAQs
Each origin of replication is matched to one host organism, enabling the DNA construct to be copied in that biological system. Including more than one origin allows the same recombinant DNA molecule to remain available during work in different hosts. This design supports bacterial amplification followed by transfer into yeast, mammalian cells, or another experimental system.
Selectable markers help researchers identify and maintain cells that contain the vector under appropriate growth conditions. Because hosts differ biologically, a marker useful in one system may not provide the same selection in another. Combining host-compatible markers therefore helps preserve the recombinant construct while it moves between organisms during an experiment.
Successful maintenance depends on matching the vector’s replication features and selectable markers to the requirements of both hosts. The relevant host must support replication from its corresponding origin and permit selection through the appropriate marker. If either host-specific component is unsuitable, researchers may be unable to amplify, preserve, or analyze the construct in that system.
Researchers commonly assemble the recombinant DNA construct in the vector, amplify it in bacteria, and then transfer it into the second host system. After introduction, host-appropriate growth conditions help maintain the construct, while the experimental system supports expression or analysis. This sequence streamlines movement of the same genetic design between biological environments.
Growth conditions provide the context in which host-specific selectable markers can identify and maintain cells carrying the desired construct. Researchers choose conditions appropriate for the organism being used and for the marker incorporated into the vector. Applying those conditions at the relevant stage helps preserve the recombinant DNA during bacterial amplification or subsequent work in another host.
They are useful when a project requires genetic material to be prepared in one organism and studied in another. Applications described for these vectors include gene cloning, protein production, functional studies, and expression analysis. Their ability to connect bacterial DNA amplification with work in yeast, mammalian cells, or other systems makes them valuable for moving experimental constructs across biological contexts.