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A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

DOI:
10.3791/54171-v

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09:16 min

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October 30, 2016

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, , , ,
1Center for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London, 2Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy,Zhengzhou University, 3School of Basic Medical Sciences, Academy of Medical Sciences,Zhengzhou University

Chapters

  • 00:05Title
  • 00:49Preparing the CV-1 Cells
  • 02:32Purification of the Modified Vaccinia Virus (VV)
  • 05:23Expanding a Purified Plaque for PCR Testing
  • 06:52Results: VV N1L Modification
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

Vaccinia virus (VV) has been widely used in biomedical research and the improvement of human health. This article describes a simple, highly efficient method to edit the VV genome using a CRISPR-Cas9 system.

Tags

Keywords: CRISPR-Cas9Mutant Vaccinia VirusViral VectorGene TherapyVaccinationCV-1 CellsTransfectionShuttle Donor VectorPlaque PurificationRFP-positive PlaquesCell LysisVirus Dilution

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