Colon Harvesting
Colon Harvesting - Related Videos
03:41
Harvesting Peritoneal Fat Depots: A Technique to Study Ovarian Cancer Colonization of Peritoneal Adipose Tissues in Mouse Models
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2023
In this video, we use a euthanized female mouse model bearing ovarian cancer. We then describe a protocol to identify and extract fat depots or adipose tissues associated with the five primary peritoneal organs to study metastatic cancer cell colonization.
03:20
Electrophysiological Recording from Colonic Afferent Nerves in an Ex Vivo Rat Colon
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2025
This video demonstrates a method to record the activity of colonic afferent nerves in an ex-vivo rat colon, cannulated at both ends and infused with saline to increase intraluminal pressure. As pressure increases, the nerve signal rises, indicating its stimulation.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
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Cited by 64 •
2016
The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
FAQs
Standardized handling reduces variation introduced by the collection process itself. Differences in isolation, separation, or preservation can alter which cellular, molecular, or microbial features remain available for examination. Keeping these factors consistent allows researchers to compare experimental groups more reliably and distinguish biological differences from differences caused by sample preparation.
Removing surrounding tissues helps focus analysis on material originating from the colon rather than on unrelated structures. This separation supports clearer examination of intestinal architecture and tissue-associated molecular findings. It also helps researchers connect observed abnormalities with colon biology, which is important when evaluating gastrointestinal pathology or responses to treatment in a study.
Colon harvesting must be matched to the feature being studied. Tissue handling should preserve cellular and molecular characteristics for histology, gene analysis, or protein analysis, whereas contents must retain relevant microbial features for microbiome investigations. The selected processing and preservation approach therefore determines which downstream observations remain interpretable.
A basic workflow begins by isolating the colon, separating it from surrounding tissues, and collecting the tissue or contents needed for analysis. The material is then processed or preserved under conditions intended to maintain the target features. This sequence creates a traceable path from collection to measurement and supports consistent comparisons among experimental groups.
Harvested colon tissue can support histology, gene analysis, and protein analysis, while colon contents can support microbiome investigations. Together, these readouts examine structure, molecular activity, and microbial features from related biological material. Using the appropriate sample type helps align the evidence with the question, whether the focus is intestinal function or disease.
In gastrointestinal research, colon harvesting enables investigators to evaluate pathology and treatment responses at the level of intestinal material. Findings from tissue or contents can be related to broader mechanisms of intestinal health rather than considered as isolated measurements. This makes the approach useful for connecting structural, molecular, and microbial observations within the same biological context.