Fraction Collector System
Fraction Collector System - Related Videos
07:11
Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification
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2026
An integrated autosampler and fraction collector (LC-mate) was developed to automate repetitive HPLC separations. Compatible with HPLC detectors that output analog signals, LC-mate enhances the performance of manually operated systems and accelerates research workflows that depend on high-performance liquid chromatography.
07:18
Experimental System of Solar Adsorption Refrigeration with Concentrated Collector
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With solar energy as the driving force, a novel adsorption refrigeration system has been developed and experimentally investigated. Water vapor and zeolite formed the working pair of the adsorption system. This manuscript describes the setup of the experimental rig, the operation procedure, and the important results.
10:56
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
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Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.
10:09
Fractional Distillation
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2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
07:57
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
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Cited by 17 •
2009
The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.
FAQs
The collection pattern is controlled by a selected trigger: a set volume, a defined time interval, or a detector signal. As effluent leaves the separation device, the system applies that rule to assign material to successive vessels. This links fraction boundaries to the separation workflow and allows researchers to recover discrete portions for later analysis.
The programmable arm or rotating rack provides the physical link between the control rule and the receiving vessels. It moves or indexes the next tube or well when the collection sequence advances, helping keep each fraction separate. This organized positioning is important when many consecutive fractions must be preserved for comparison or downstream processing.
Volume-, time-, and signal-based collection do not describe the effluent in the same way. A volume rule groups material by liquid amount, a time rule divides the run into temporal intervals, and a detector signal can determine collection according to an observed separation event. Selecting among them aligns fraction boundaries with the experimental objective.
Automation changes fraction handling rather than the underlying separation. Once the column or other liquid-phase device produces effluent, programmed collection reduces the need for repeated manual transfers. The resulting workflow improves reproducibility and reduces sample loss, which is especially valuable when multiple fractions must undergo comparable biological analyses.
A typical setup coordinates the separation device, the collection vessels, and the collector’s programmed rule. Researchers choose whether fractions will be assigned by volume, time, or detector signal, then allow the arm or rack to direct successive effluent portions into tubes or wells. This creates an ordered set for subsequent examination.
Collected fractions can be examined directly in downstream workflows or retained for further processing. The overview identifies electrophoresis, spectroscopy, and biochemical characterization as compatible uses. Because each portion remains associated with its collection position, researchers can compare fractions and determine which portions merit additional analysis.
Within biology, the system is useful when a separation produces fractions containing proteins, nucleic acids, metabolites, or cellular components. Its value is not limited to one analyte class: the same collection approach preserves separate portions so different biological materials can be analyzed or processed after chromatography or another liquid-phase separation.