Sonication Treatment
Sonication Treatment - Related Videos
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
03:22
Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
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2023
This video describes the selective damage of human leukemia cells by using low frequency ultrasound. This application of low frequency ultrasound in a preclinical in vitro condition demonstrates that cell destruction can be attained in order to examine and compare the effects of sonication on leukemia and normal cells.
10:10
Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
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Cited by 5 •
2014
Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.
FAQs
Acoustic cavitation provides the main mechanical force. Rapid pressure changes create bubbles in the liquid, and their collapse produces localized shear forces. Those forces can rupture cell membranes, detach tissue components, and reduce particle aggregates. This mechanism allows the same treatment to support biological disruption, dispersion, or mixing, depending on the sample and preparation goal.
Power, duration, temperature, and sample volume define the treatment conditions. These variables influence how extensively membranes, tissue-associated components, or aggregates are disrupted. Temperature requires particular attention because acoustic energy can produce heating, while excessive thermal exposure may affect sensitive biomolecules. Controlling the full set of parameters helps limit unwanted heating and tailor the treatment to the sample.
Effective treatment should release the desired cellular contents without compromising sensitive biomolecules through uncontrolled heating or excessive disruption. Researchers therefore adjust the acoustic exposure rather than treating disruption as the only objective. This balance matters when preparing proteins, DNA, or organelles for downstream analysis, because the quality of the released material affects the usefulness of the resulting sample.
Researchers first place the biological material in a liquid sample suitable for acoustic treatment, then use either an ultrasonic probe or bath to transfer energy into it. They select and control power, duration, temperature, and volume during exposure. After disruption, dispersion, or homogenization, the prepared material can proceed to the intended downstream molecular or biological analysis.
The method is useful when biological samples require cell lysis, intracellular-content release, homogenization, or reduction of particle aggregation. It can help make proteins, DNA, and organelles accessible for subsequent analysis and can detach components from tissue material. These capabilities make sonication relevant to sample preparation across molecular biology workflows where the starting material must be physically disrupted or dispersed.
Researchers can examine whether the treatment produced the intended physical preparation, such as disrupted cell membranes, detached tissue components, reduced particle aggregates, or a more homogenized suspension. They can also determine whether proteins, DNA, or organelles were released for downstream analysis. Interpreting these outcomes alongside the applied power, duration, temperature, and volume helps connect sample quality with treatment conditions.