Stromal Fibroblasts
Stromal Fibroblasts - Related Videos
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
0 Views •
Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
13:01
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
0 Views •
Cited by 14 •
2011
In this report, we demonstrate a system to isolate and culture donor cells from the mouse mammary gland, and orthotopically transplant these cells in recipient mice to analyze stromal: epithelial interactions during mammary tumor development.
09:43
Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
0 Views •
Cited by 1 •
2025
This protocol provides a comprehensive procedure to isolate, expand, and immortalize fibroblasts from radical prostatectomies. Moreover, it describes assays developed to assess the functional effects of fibroblast-tumor cell crosstalk, taking advantage of both treatment with conditioned medium and co-culturing.
06:02
Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens
0 Views •
Cited by 17 •
2016
Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.
12:42
Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
0 Views •
Cited by 20 •
2012
We describe methodologies for establishing in vitro heterotypic three-dimensional models comprising ovarian fibroblasts and normal ovarian surface or ovarian cancer epithelial cells. We discuss the use of these models to study stromal-epithelial interactions that occur during ovarian cancer development.
FAQs
Their activity changes the amount and organization of extracellular matrix components, including collagen and fibronectin. These changes can alter tissue stiffness and the physical environment surrounding other cells. Because local cells respond to that environment, shifts in matrix organization may influence cellular behavior during normal tissue maintenance, repair, or disease-associated remodeling.
Secreted signaling molecules allow stromal fibroblasts to communicate with nearby cells rather than acting only through matrix production. This communication can regulate local inflammation, repair, and remodeling. Studying these signals helps explain how fibroblast activity coordinates tissue responses and how altered communication may contribute to persistent or abnormal changes in the cellular environment.
In fibrosis and tumor development, changes in stromal fibroblast activity can modify extracellular matrix organization, tissue stiffness, and local signaling. These effects may create a tissue environment that differs from normal repair or maintenance. Researchers therefore examine fibroblast behavior as part of broader disease mechanisms involving abnormal remodeling and altered interactions among cells.
During wound healing, stromal fibroblasts are relevant because their matrix production and organization support tissue repair and remodeling. Their signaling also affects nearby cells and local inflammation. Research focuses on how these activities help restore tissue structure, while recognizing that changes in the same processes can produce excessive remodeling or contribute to fibrosis.
Researchers can assess how fibroblast activity affects extracellular matrix organization, tissue stiffness, inflammation, repair, and remodeling. They can also investigate consequences for nearby cellular behavior and communication. Together, these outcomes connect cellular activity with tissue-level changes, making stromal fibroblasts useful for studying both normal biology and disease-associated processes.
Their influence extends across tissue structure, local signaling, and remodeling, so stromal fibroblasts connect cellular behavior with broader tissue outcomes. In tissue regeneration, this relationship informs studies of repair and restoration. In disease research, it supports investigation of fibrosis and tumors, where abnormal remodeling or altered tissue stiffness may affect disease development and potential therapeutic strategies.