Vacuum Aspirator Use
Vacuum Aspirator Use - Related Videos
06:20
Proper Operation of Vacuum Based Equipment
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2023
Source: Robert M. Rioux, Ajay Sathe & Zhifeng Chen, Pennsylvania State University, University Park, PA Vacuum is required for a number of laboratory procedures. This is most routinely achieved in the laboratory by the use of vacuum pumps. In addition to working at low pressures, vacuum pumps can also be used to enable rapid changing of the atmospheres in a reactor or flask by evacuation and backfilling.
06:43
Fluid Aspiration from Mouse Glomeruli: A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture
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2023
This video showcases fluid aspiration from mouse glomeruli using the two-photon mediated micropuncture technique. The technique provides access to the living mouse’s glomeruli and thus helps gain insights into renal physiology.
09:26
Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies
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Cited by 6 •
2009
Practicing of fine needle aspiration biopsies (FNAB) by trainees is relatively challenging, due to the lack of an easily available, appropriate lesion. Preparation of an AV phantom lesion for practicing the FNAB procedure and mastering proficiency is relatively easy.
02:43
Fly Aspirator Tube: A Simple Tool to Transfer Live Drosophila
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2023
This video describes one method to build a fly aspirator, which can be used to transfer insects between enclosures without anesthesia. The aspirator in the example protocol was prepared for an experiment to quantify individual grooming...
07:10
Femoral Bone Marrow Aspiration in Live Mice
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Cited by 18 •
2014
This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.
FAQs
The vacuum source lowers pressure within the connected tubing, creating a pressure difference that draws liquid, gas, or particulate material toward the collection vessel. Because movement depends on this pressure gradient, the tubing pathway and vessel must remain properly connected. This mechanism enables controlled removal during procedures without manually transferring material between containers.
Collection vessels receive the material removed from a sample or work area, while protective traps help prevent waste from reaching the vacuum pump. Together, they contain biological or other laboratory materials, reduce contamination of connected equipment, and support safer handling. Their use also helps preserve a cleaner working environment during repeated aspiration tasks.
Controlled suction removes unwanted media, rinsing solutions, gases, or particulate material without requiring direct handling of each portion. This can make sequential steps more consistent and limit exposure to biological materials. In cell culture and tissue-processing workflows, predictable removal helps maintain organized conditions and supports cleaner, more reproducible experimental handling.
A basic setup includes the vacuum source, tubing, a collection vessel, and a protective trap positioned to help shield the pump from collected material. Connections should form a continuous pathway from the work area to the vessel and vacuum source. This arrangement allows the system to collect removed material while limiting contamination of surrounding equipment.
During cell culture work, aspiration can remove spent culture media or rinsing solutions from vessels before the next processing step. The liquid travels through tubing into a collection container rather than being transferred manually. This application reduces handling of biological materials, supports orderly workflow transitions, and helps maintain cleaner conditions around the culture area.
Vacuum aspirator use is useful when tissue-processing steps require clearing samples or removing accumulated liquids and particulate material. The system directs unwanted material into a contained vessel, while a protective trap helps prevent transfer toward the pump. By supporting efficient clearing and liquid-waste collection, aspiration can help keep processing areas organized and reduce exposure during routine work.