Vacuum Chamber Setup
Vacuum Chamber Setup - Related Videos
07:49
Development of an Experimental Setup for the Measurement of the Coefficient of Restitution under Vacuum Conditions
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Cited by 11 •
2016
The coefficient of restitution is a parameter that describes the loss of kinetic energy during collision. Here, a free-fall setup under vacuum conditions is developed to be able to determine the coefficient of restitution parameter for particles in micrometer range with high impact velocities.
03:03
Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration
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2026
Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.
09:23
A Low Cost Setup for Behavioral Audiometry in Rodents
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Cited by 17 •
2012
A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.
06:20
Proper Operation of Vacuum Based Equipment
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2023
Source: Robert M. Rioux, Ajay Sathe & Zhifeng Chen, Pennsylvania State University, University Park, PA Vacuum is required for a number of laboratory procedures. This is most routinely achieved in the laboratory by the use of vacuum pumps. In addition to working at low pressures, vacuum pumps can also be used to enable rapid changing of the atmospheres in a reactor or flask by evacuation and backfilling.
07:33
A Customizable Chamber for Measuring Cell Migration
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Cited by 2 •
2017
This protocol details a customizable method to measure cell migration in response to chemoattractants that may also be used to determine the diffusion rate of a drug out of a polymer matrix.
FAQs
These components determine whether the chamber reaches and maintains the intended low-pressure condition. Seals limit unwanted gas entry, valves regulate evacuation and controlled venting, and pressure gauges show how the internal pressure changes during treatment. Coordinating them helps create stable, repeatable conditions rather than relying only on pump operation, which is important when biological samples require consistent exposure.
The effect of reduced pressure depends on how long the sample remains exposed and on the temperature during treatment. Changing any one of these conditions can alter moisture removal or the handling of biological materials. Controlled settings help limit unintended sample effects and make results more reproducible across drying, degassing, embedding, or infiltration procedures.
Air removal clears trapped gas, whereas vacuum-assisted infiltration uses pressure reduction as part of preparing a material to enter spaces within a biological specimen or embedding material. The distinction matters because infiltration requires coordinated treatment rather than vacuum exposure alone. Proper control can improve preparation consistency by supporting more complete replacement of trapped air during the procedure.
A basic workflow places the sample or solution in the sealed chamber, checks the seals and pressure-monitoring components, reduces pressure with the pump, and observes the pressure during the planned treatment. Afterward, the chamber is vented in a controlled manner and the sample is removed or processed. Pressure, time, and temperature should be recorded for repeatability.
Applications include drying tissues or specimens, removing trapped air from solutions and embedding materials, and supporting vacuum-assisted infiltration. These uses rely on different outcomes, so the operating conditions should match the task rather than follow one universal setting. In each case, controlled evacuation and re-pressurization help preserve the intended preparation and improve procedural consistency.
Researchers should monitor the pressure indicated by the gauge, the duration of low-pressure exposure, the temperature, and the way the chamber is returned to normal pressure. They should also assess whether seals and valves maintain the selected conditions. Tracking these factors helps identify inconsistent treatment and supports more reliable drying, degassing, embedding, or infiltration results.