Biolayer Punch Electrode

A biolayer punch electrode is a specialized electrode designed to create electrical contact through a thin biological layer, enabling measurements from cells or tissues that are otherwise difficult to access. Its pointed or punched geometry penetrates the target layer while the conductive surface couples ionic activity in the biological material to an external recording circuit. This design supports localized electrophysiological measurements, electrical stimulation, and characterization of barrier or membrane properties. In biology, biolayer punch electrodes can help researchers examine cell signaling, tissue function, and bioelectrical responses with greater spatial specificity than surface-contact electrodes.

Biolayer Punch Electrode - Related Videos

Research

JoVE EoE - Biomolecular Interaction Detection Techniques

Biolayer Interferometry Technology to Detect Interactions between Ligand and Analyte

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2025

In this video, we demonstrate biolayer interferometry (BLI) technology to detect the intermolecular interactions between ligands and target analytes in real-time. BLI measures changes in the interference pattern of white light reflected off a layer of immobilized ligands on a biosensor surface as they interact with analytes in solution.

Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy

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2025

This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.

Picoinjection of Microfluidic Drops Without Metal Electrodes

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Cited by 2 •

2014

We have developed a technique for picoinjecting microfluidic drops that does not require metal electrodes. As such, devices incorporating our technique are simpler to fabricate and to use.

Education

JoVE Core - Chemistry

Standard Electrode Potentials

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2020

On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...

Fabrication of Amperometric Electrodes

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Cited by 8 •

2009

This protocol describes how to generate carbon fiber electrodes. The electrodes are subsequently used to detect catecholamine release from vesicles with carbon fiber amperometry.

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