3d Organotypic Culture
3d Organotypic Culture - Related Videos
03:53
Laser Microdissection: A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model
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2023
The video describes the laser dissection of the 3D organotypic co-culture model with micron-level precision. In 3D organotypic models, stromal constituents such as fibroblasts are 3-dimensionally co-cultured with cancer epithelial cells. The 3D model serves as an experimental tool that enables invasion and cancer-stroma interactions to be studied in near-physiological conditions.
04:21
Organotypic 3D Skin Culture: A Cassette-based In Vitro Culture Technique to Regenerate Human Epidermis on Acellular Dermis
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2023
This video protocol describes a cassette-based in vitro culture technique to generate human organotypic skin using epidermal keratinocytes and devitalized human dermis. They mimic the function and physiology of their in vivo tissue counterparts.
07:48
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
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Cited by 32 •
2013
The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.
06:47
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
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Cited by 15 •
2015
Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.
09:50
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Cited by 41 •
2015
The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
FAQs
The extracellular matrix acts as more than a physical scaffold in 3D organotypic culture. Its arrangement supports cell-matrix interactions and helps preserve spatial relationships among cells. Those relationships can influence how tumor cells organize and behave, allowing researchers to examine growth or invasion in a tissue-like setting rather than viewing cells as an isolated layer.
Spatial gradients create differences in the local environment across the model, an aspect largely absent from conventional two-dimensional culture. These variations let investigators consider how position and surrounding tissue conditions relate to tumor growth, invasion, and treatment response. The model therefore connects cellular behavior with the architecture in which that behavior occurs.
Compared with two-dimensional culture, the model retains three-dimensional architecture, cell-cell contacts, cell-matrix interactions, and spatial gradients. Compared with an animal model, it offers a more controlled laboratory system. This intermediate position helps cancer researchers study tumor biology with greater tissue relevance than simplified cultures while remaining more experimentally controlled than complex animal models.
To establish a 3D organotypic culture, researchers arrange the selected cells within or on a supportive extracellular matrix and maintain the intended three-dimensional organization. The design should preserve opportunities for cell-cell and cell-matrix interactions. In cancer studies, this setup provides the structural context needed to examine tumor growth, invasion, or responses to treatment.
Researchers can apply these cultures to questions about how tumor cells grow, invade surrounding tissue, and interact with stromal components. Because the model preserves tissue architecture and a local microenvironment, it helps link cancer-cell behavior to neighboring support cells or structures. These features make it useful for investigating mechanisms of tumor biology under controlled laboratory conditions.
Treatment studies can use the model to examine how tumors respond within a three-dimensional tissue context rather than in a flat cell layer. Investigators can compare cancer-related behavior and treatment response under the same controlled model conditions. Results may help evaluate potential therapies and identify effects that could be missed when tissue architecture and spatial interactions are absent.