Journal
/
Developmental Biology
/
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

DOI:
10.3791/52614-v

•

06:47 min

•

July 30, 2015

•

, , , ,
1Division of Developmental Immunology,German Cancer Research Center (DKFZ), 2Genetics of Skin Carcinogenesis,German Cancer Research Center (DKFZ)

Chapters

  • 00:05Title
  • 01:163D Organotypic Co-cultures (OTCs)
  • 02:46mTEC Co-culture
  • 05:14Results: Growth Patterns and Proliferation of mTECs within the OTCs
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.

Tags

Keywords: 3D Organotypic Co-culture ModelMedullary Thymic Epithelial Cells (mTECs)Promiscuous Gene ExpressionT Cell DevelopmentSelf-toleranceRANKLFoxN1AireCortical Thymic Epithelial Cells (cTECs)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52614?article_type=v

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
check_url/52614?article_type=v

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
check_url/52614?article_type=v

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
check_url/52614?article_type=v

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
check_url/52614?article_type=v

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
check_url/52614?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/52614?article_type=v

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
check_url/52614?article_type=v

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
check_url/52614?article_type=v

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
check_url/52614?article_type=v

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Read Article