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Developmental Biology

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JoVE Journal Developmental Biology
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
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3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Article DOI: 10.3791/52614
July 30th, 2015 •
*1, *2, 2, 2, 1
1Division of Developmental Immunology, German Cancer Research Center (DKFZ), 2Genetics of Skin Carcinogenesis, German Cancer Research Center (DKFZ)
* These authors contributed equally

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Summary
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July 30th, 2015

Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.

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