Cell Repellent Plates
Cell Repellent Plates - Related Videos
10:43
High-throughput Identification of Bacteria Repellent Polymers for Medical Devices
0 Views •
Cited by 2 •
2016
A high-throughput microarray method for the identification of polymers which reduce bacterial surface binding on medical devices is described.
02:49
High-Throughput Identification of Bacteria-Repellent Polymers via Microarray Screening
0 Views •
2025
Source: Venkateswaran, S., et al. High-throughput Identification of Bacteria Repellent Polymers for Medical Devices. J. Vis. Exp. (2016)This video demonstrates a high-throughput method to identify bacteria-repellent polymers using a microarray slide exposed to mixed bacterial cultures. Bacterial binding is assessed by DAPI fluorescence, where reduced signal indicates polymers with minimal surface attachment.
03:38
Preparation of a Microfluidic Plate with Bacterial Cells
0 Views •
2025
Source: Sutlief, A. L., et al. Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System. J. Vis. Exp. (2019).This video demonstrates the preparation of a microfluidic plate for shear stress studies in bacterial cultures. After priming the channels with media, bacterial cells are introduced into the experimental channel and incubated for attachment. Residual media and culture are removed to prepare the system for subsequent shear...
11:04
Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
0 Views •
Cited by 4 •
2013
This study used a multi-well plate microfluidic system, significantly increasing throughput of cell rolling studies under physiologically relevant shear flow. Given the importance of cell rolling in the multi-step cell homing cascade and the importance of cell homing following systemic delivery of exogenous populations of cells in patients, this system offers potential as a screening platform to improve cell-based therapy.
05:46
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
0 Views •
Cited by 7 •
2017
Here, we present a protocol to perform a semi-high throughput cell migration assay on a 96-well cell culture plate. This protocol is a fast, simple and economical method to create consistent scratch wounds on a cell monolayer.
FAQs
The coating is central to how these plates alter cell behavior. By limiting protein adsorption, it reduces the surface-associated conditions that normally support cell attachment and spreading. Cells consequently remain free-floating and can contact neighboring cells, allowing controlled formation of cellular aggregates such as tumor spheroids rather than a surface-bound layer.
Limiting protein adsorption helps preserve the intended non-adhesive surface during culture. If cells readily attached through surface-associated proteins, they could spread across the vessel instead of remaining in suspension. Maintaining this separation from the plate directs attention toward cell-cell organization and aggregate formation, which are central to three-dimensional cancer models.
Conventional two-dimensional cultures encourage cells to spread across a surface, whereas these plates support free-floating organization. The resulting three-dimensional assemblies can represent cell-cell interactions, nutrient gradients, and treatment responses in a way that differs from many flat cultures. This comparison helps explain why the format is valuable for more physiologically relevant tumor models.
Cells are placed in vessels with non-adhesive surfaces and maintained under controlled culture conditions so they remain suspended while aggregates develop. The essential procedural objective is to prevent spreading across the plate, then use the resulting three-dimensional assemblies for further study. This approach supports examination of tumor spheroid formation and related responses.
They are useful when a study requires tumor spheroids or another three-dimensional model rather than a flat, surface-attached culture. Cancer researchers can apply them to investigate tumor biology, evaluate anticancer compounds, and develop systems intended to reflect relevant cell-cell interactions, nutrient gradients, and treatment responses more closely than many two-dimensional models.
These assemblies allow researchers to examine how cells organize collectively and how that organization relates to nutrient gradients and responses to treatment. In practice, the models can support tumor-biology studies and anticancer compound evaluation. Their value lies in providing experimental observations from a three-dimensional context, complementing findings obtained with two-dimensional cultures.