Decellularized Tongue Ecm
Decellularized Tongue Ecm - Related Videos
04:51
Fabrication of Extracellular Matrix from Mouse Model: An Effective Procedure to Prepare Decellularized Tongue Extracellular Matrix from Murine Tongue
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2023
This video demonstrates the preparation of the murine tongue extracellular matrix by using the decellularization method, which involves exposing the harvested tongue to a series of physical, chemical, and enzymatic treatments. The resulting TEM scaffold retains its native extracellular matrix composition, removing the cellular components.
02:31
Extracellular Matrix-derived Foam Fabrication via Lyophilization: A Procedure to Generate Biological Scaffolds from ECM of Decellularized Tissues
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2023
This video describes the method for synthesizing pure ECM-derived foams without the need for chemical crosslinking. These ECM-derived foams find applications as advanced 3D in vitro cell culture models or as pro-regenerative bioscaffolds.
05:17
Cardiopulmonary Complex Decellularization: A Technique for Decellularizing Heart and Lungs from Murine Model
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2025
This video demonstrates a method of producing decellularized extracellular matrix or ECM scaffolds by applying antegrade perfusion of detergents through the vasculature. The structural ECM scaffolds can then be used to analyze ECM remodeling associated with cardiopulmonary disease.
02:22
Cryomilling of Decellularized Tissue: A Technique to Obtain Fine Extracellular Matrix Powder
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2023
In this video, we present the protocol to obtain finely ground extracellular matrix powder from lyophilized decellularized tissue by cryomilling. The powdered ECM retains its biochemical composition and can be used for microcarrier synthesis.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
FAQs
The process must remove cellular material without destroying the matrix features that give the scaffold biological meaning. Chemical or enzymatic treatments disrupt cell membranes and degrade nucleic acids, while controlled processing aims to retain structural proteins, glycosaminoglycans, and native matrix architecture. Preserving these components helps maintain signals that affect tumor-cell attachment, growth behavior, and interactions with the surrounding tissue.
Structural proteins, glycosaminoglycans, and the organization of the matrix provide distinct biochemical and physical cues. These features can influence how oral or tongue tumor cells attach to the scaffold and respond to their surroundings. Because the material retains tissue-specific characteristics, it can support investigation of how local extracellular conditions shape cancer-cell behavior rather than examining tumor cells in isolation.
Tumor cells respond not only to neighboring cells but also to the biochemical and mechanical environment surrounding them. A tongue-derived matrix supplies cues associated with the tissue being studied, allowing experiments to examine microenvironment-dependent behavior. This context is particularly relevant when investigators want to explore invasion or treatment responses in a three-dimensional setting that better reflects tissue-specific conditions.
Preparation centers on treating tongue tissue with chemical or enzymatic methods that disrupt cell membranes and degrade nucleic acids. Processing is then controlled to favor removal of cellular components while retaining structural proteins, glycosaminoglycans, and matrix architecture. The resulting scaffold can be used as a three-dimensional culture framework, provided that the treatment preserves the features needed to influence cell attachment and behavior.
Investigators can use the scaffold to support three-dimensional culture of oral or tongue tumor cells. This arrangement creates an experimental setting for examining tumor-microenvironment interactions, including how cells attach to and respond to tissue-specific matrix cues. It can also help researchers study invasion and compare cellular behavior under conditions that include a tongue-derived extracellular framework.
A decellularized tongue ECM model can support studies of tumor invasion, drug responses, and interactions between cancer cells and their surrounding matrix. These outcomes help connect cellular behavior with tissue-specific biochemical and mechanical cues. In cancer research, the approach is therefore useful for asking whether a tongue-like extracellular environment changes how oral or tongue tumor cells behave during disease-related experiments.