Body Centered Cubic

Body Centered Cubic (BCC) is a crystal structure in which atoms occupy the eight corners of a cubic unit cell and one additional atom sits at its center, making this lattice geometry important for interpreting the properties of crystalline materials. The arrangement gives an effective two atoms per unit cell and a coordination number of eight, because the body-centered atom is nearest to atoms at the surrounding corners. Its packing efficiency is about 68%, lower than close-packed structures, leaving more open space within the lattice. These features help relate atomic arrangement to density, diffusion, thermal expansion, and deformation in materials such as iron, chromium, and tungsten.

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Education

JoVE Core - Chemistry

Lattice Centering and Coordination Number

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2020

The structure of a crystalline solid, whether a metal or not, is best described by considering its simplest repeating unit, which is referred to as its unit cell. The unit cell consists of lattice points that represent the locations of atoms or ions. The entire structure then consists of this unit cell repeating in three dimensions. The three different types of unit cells present in the cubic lattice are illustrated in Figure 1. Types of Unit Cells Imagine taking a large number of identical...

Research

JoVE Journal - Medicine

Whole-Kidney Three-Dimensional Staining with CUBIC

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Cited by 5 •

2022

The present protocol describes a tissue clearing method and whole-mount immunofluorescent staining for three-dimensional (3D) kidney imaging. This technique can offer macroscopic perspectives in kidney pathology, leading to new biological discoveries.

Research

JoVE Journal - Biochemistry
Free Sample

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

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Cited by 20 •

2016

We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

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Cited by 13 •

2016

Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

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Cited by 9 •

2016

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

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