Glycerol Droplets

Glycerol droplets are discrete volumes of glycerol, a highly viscous, hygroscopic polyol, formed or maintained as microscale or larger liquid compartments for chemical study and handling. Their behavior depends on viscosity, surface tension, density, and water exchange: glycerol attracts moisture from the surrounding environment, while differences in interfacial tension and fluid properties govern droplet formation, stability, and coalescence. In chemistry, glycerol droplets provide controlled environments for reactions, solute concentration, sample preservation, and microfluidic experiments. Studying their transport and phase behavior supports droplet-based analysis, liquid handling, and the design of systems that require stable, compositionally tunable microscopic volumes.

Glycerol Droplets - Related Videos

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JoVE EoE - Bacterial Growth and Techniques

Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide

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2025

Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.

Archimedes-Based Glycerol Displacement for Electrode Porosity Measurement in Lead-Acid Batteries

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2026

Here, we present a simple and low-cost glycerol displacement method based on Archimedes' principle for measuring lead-acid battery electrode porosities. The method demonstrates good reproducibility and is safer than traditional techniques, requiring low-hazard chemicals and equipment. Combining the technique with microstructural analysis by X-ray diffraction provides information on battery electrode properties and degradation.

Title Cell Encapsulation by Droplets

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Cited by 4 •

2007

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JoVE Journal - Bioengineering
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Fluorescence detection methods for microfluidic droplet platforms

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Cited by 20 •

2011

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

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Cited by 9 •

2014

Synthesis and fabrication of electrospun long fibers spanning a larger deposit area via a newly designed collector from a novel biodegradable polymer named poly(glycerol-dodecanoate) (PGD) was reported. The fibers were able to support the growth of cells derived from mouse pluripotent stem cells.

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