Clonal Hematopoietic Analysis
Clonal Hematopoietic Analysis - Related Videos
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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Cited by 11 •
2014
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.
14:42
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
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Cited by 11 •
2008
We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta.
06:40
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
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Cited by 10 •
2015
Cell organization of craniofacial bones has long been hypothesized but never directly visualized. Multi-spectral cell labeling and in vivo live imaging allows visualization of dynamic cell behavior in zebrafish lower jaw. Here, we detail the protocol to manipulate Zebrabow transgenic fish and directly observe cell intercalation and morphological changes of chondrocytes in the Meckel’s cartilage.
09:32
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
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Cited by 11 •
2018
Here we present methodology for the clonal analysis of hematopoietic stem cell precursors during murine embryonic development. We combine index sorting of single cells from the embryonic aorta-gonad-mesonephros region with endothelial cell co-culture and transplantation to characterize the phenotypic properties and engraftment potential of single hematopoietic precursors.
12:03
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Cited by 18 •
2012
A method to analyse the distribution of bone marrow hematopoietic progenitors in flow cytometry as well as to efficiently isolate highly purified hematopoietic stem cells (HSCs) is described. The isolation procedure is essentially based on magnetic enrichment of c-Kit+ cells and cell sorting to purify HSCs for cellular and molecular studies.
FAQs
Marker patterns provide a way to compare cells within a developing blood population. When multiple cells carry the same genetic marker, naturally occurring mutation, or experimental barcode, their shared pattern can support reconstruction of lineage relationships. Differences between patterns help distinguish descendant groups and identify whether particular clones have expanded relative to other blood-forming populations.
This balance determines whether a stem or progenitor cell maintains the blood-forming population or produces more specialized descendants. Following individual clones lets researchers examine how these outcomes are distributed across blood lineages and how the balance changes over time. The resulting patterns connect cellular behavior with developmental decisions rather than treating the population as uniform.
Tracking marker patterns across successive cell populations can reveal shifts in which clones contribute to blood production. A clone may remain limited, contribute to several lineages, or become disproportionately represented, indicating clonal expansion. Comparing these patterns at different stages helps researchers study changing developmental behavior and the temporal organization of hematopoiesis.
A typical analysis first identifies individual blood-forming stem or progenitor cells through genetic markers, naturally occurring mutations, or experimental barcodes. Researchers then track those identifiers in descendant hematopoietic cells and compare marker patterns across the resulting populations. These comparisons support lineage reconstruction, assessment of clonal expansion, and evaluation of contributions to different blood lineages.
The method is useful when researchers need to determine which blood-forming populations contribute to regeneration or to an abnormal cell population. By following clone-associated markers, they can assess whether particular populations expand and examine their cellular origins. This connects observable blood-cell changes with the developmental history of the cells that produced them.
In developmental biology, the approach links individual stem and progenitor cell behavior to the emergence of diverse blood lineages. It helps clarify how self-renewal and differentiation decisions are organized during development and how those decisions change with time. The same lineage information also provides context for studying regeneration, hematopoietic disorders, and abnormal blood populations.