Human Amniotic Epithelial Cells
Human Amniotic Epithelial Cells - Related Videos
10:00
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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Cited by 8 •
2019
This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Cited by 3 •
2017
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
FAQs
Their epithelial barrier activity contributes to maintaining the fetal environment by supporting the boundary formed by the amniotic membrane. In addition, these cells release signaling molecules, so they may influence local developmental communication rather than acting only as a physical lining. Studying both properties helps researchers examine how membrane-associated cells participate in fetal tissue organization.
Defined developmental cues are important because the cells’ plasticity allows them to respond to those cues and adopt characteristics of other cell types. This response provides a way to investigate differentiation as a regulated process, rather than treating epithelial identity as fixed. The resulting model can connect amnion biology with broader questions about developmental cell-state changes.
Interactions between fetal tissues and surrounding membranes can be examined through the cells’ combined barrier and signaling activities. Their position at the amniotic interface makes them relevant to questions about how neighboring tissues communicate and how membrane-associated conditions support development. This perspective extends analysis beyond isolated differentiation markers to relationships among cells, tissues, and their developmental environment.
They provide an accessible cellular context for examining how the amnion forms and how its cells differentiate. Researchers can also use them to relate changes in epithelial characteristics to interactions between fetal tissues and surrounding membranes. This combination makes the model useful for linking cell-level behavior with the larger developmental organization of the amniotic environment.
Accessibility can make these cells practical for research, while low immunogenicity broadens their relevance beyond basic developmental studies. Together, these features support investigation of tissue repair, cell-based therapies, and regenerative medicine. They matter not only because of what the cells do biologically, but also because they influence how readily researchers can consider repair-oriented and therapeutic applications.
In tissue repair and regenerative medicine, investigators can examine whether the cells’ plasticity and signaling activity are relevant to restoring or supporting damaged tissues. Cell-based therapy research likewise benefits from considering their low immunogenicity alongside their developmental properties. These applications extend the cells’ value from modeling fetal membranes to evaluating broader repair-oriented strategies.