Ex Situ Samples
Ex Situ Samples - Related Videos
11:20
In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions
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Cited by 1 •
2017
This article describes a setup and method for the in situ visualization of oil samples under a variety of temperature and pressure conditions that aim to emulate refining and upgrading processes. It is primarily used for studying isotropic and anisotropic media involved in the fouling behavior of petroleum feeds.
08:17
Sample Preparation for In Situ Cryotomography of Mammalian Cells
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Cited by 1 •
2023
This method provides an accessible and flexible protocol for the preparation of electron microscopy (EM) grids for in situ cellular cryotomography and correlative light and electron microscopy (CLEM).
07:24
In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
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Cited by 5 •
2018
The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
11:59
In Situ Characterization of Boehmite Particles in Water Using Liquid SEM
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Cited by 5 •
2017
We present a procedure for real-time imaging and elemental composition analysis of boehmite particles in deionized water by in situ liquid Scanning Electron Microscopy.
FAQs
Removing a specimen from service conditions makes it possible to control the laboratory environment and repeat measurements under defined settings. That control helps engineers separate material or device behavior from changing operating influences, while detailed preparation supports consistent characterization. The trade-off is that removal may alter environmental effects or transient behavior, so results are strongest when interpreted alongside in situ monitoring.
Ex situ analysis supplies controlled, detailed measurements after a specimen has been isolated, whereas in situ monitoring preserves the specimen’s operating context. The two approaches therefore answer different engineering questions: laboratory characterization can reveal structure, composition, mechanical response, or performance in repeatable conditions, while in situ observations help retain service-related effects. Combining their findings improves interpretation without treating either method as complete alone.
Sample preparation, isolation from service conditions, and the selected laboratory settings can influence what engineers observe. Preparation determines how the specimen is presented for examination, while defined conditions improve repeatability across measurements. Instrument choice also matters because different dedicated instruments support characterization of structure, composition, mechanical response, or performance. Engineers must therefore relate each result to its preparation and testing context.
An engineering workflow begins by collecting or fabricating a specimen, then removing and isolating it from the operating environment. The sample is prepared under controlled laboratory conditions before engineers select dedicated instruments for characterization. Measurements may address structure, composition, mechanical response, or performance. Recording the preparation and test conditions helps place the resulting data in context and supports repeatable comparisons.
They are useful when engineers need detailed evidence for materials selection, failure analysis, quality control, or validation of manufacturing processes. A controlled specimen provides focused information about a material or device during examination. These applications connect laboratory measurements to design decisions, process checks, and investigations of whether manufactured results meet intended requirements.
Characterization can describe a specimen’s structure and composition, measure its mechanical response, or assess aspects of device performance. The resulting data support comparisons among materials, examination of damaged or failed specimens, and evaluation of manufacturing processes. Because the measurements occur under defined laboratory conditions, engineers can obtain repeatable evidence, while still considering that service-related or transient behavior may not be fully preserved.