Gel Water Volume
Gel Water Volume - Related Videos
07:28
A Small Volume Procedure for Viral Concentration from Water
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Cited by 5 •
2015
An approach was developed for identifying optimal viral concentration conditions for small volume water samples using spikes of human adenovirus. The techniques described here are used to identify concentration parameters for other viral targets, and applied to large-scale viral concentration experimentation.
09:54
A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry
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Cited by 13 •
2015
The goal of this procedure is to demonstrate the reproducibility and adaptability of using a microtiter plate format for microalgal screening. This rapid screen combines WATER-Pulse-Amplitude-Modulated (WATER-PAM) fluorometry to measure photosynthetic yield as an indicator of Photosystem II (PSII) health with small volume bacterial-algal co-cultures.
06:30
Gel Purification
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2023
Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, washing and eluting from a silica column. DNA is believed to bind to silica in the presence of high salt via a salt bridge. Following binding, DNA is washed of impurities and eluted under low salt conditions disrupting this interaction. This video goes through a step-by-step, generalized procedure for cutting out a band from the gel, gel...
13:26
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
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Cited by 9 •
2015
This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.
12:50
Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
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Cited by 24 •
2014
A protocol involving integrated concentration, enrichment, and end-point colorimetric detection of foodborne pathogens in large volumes of agricultural water is presented here. Water is filtered through Modified Moore Swabs (MMS), enriched with selective or non-selective media, and detection is performed using paper-based analytical devices (µPAD) imbedded with bacterial-indicative colorimetric substrates.
FAQs
Swelling results from osmotic forces drawing water into the network when surrounding conditions favor liquid uptake. As water enters, the polymer or particle framework resists expansion through its elasticity. Gel water volume therefore increases until the inward osmotic drive and outward network resistance reach balance. This equilibrium helps explain why gels change size while retaining structural integrity.
Network elasticity provides the restoring force that limits expansion as water enters a gel. Its interaction with osmotic uptake determines how much the structure can swell rather than allowing indefinite growth. Because this balance affects final dimensions, elasticity becomes important when engineers evaluate dimensional stability and mechanical behavior in water-containing materials.
Temperature, pressure, and solute concentration can shift the balance governing water uptake and retention. Changes in temperature may alter swelling, while pressure can oppose expansion. Solute concentration outside the network also influences osmotic forces. Consequently, gel water volume is not necessarily a fixed material constant; its value depends on the conditions used for evaluation.
Measurement connects the amount of retained water with observable engineering performance. By determining gel water volume, investigators can characterize swelling and examine its relationship to porosity, permeability, and dimensional stability. This information helps distinguish materials or operating conditions that produce different degrees of expansion and fluid transport, providing a basis for selecting gels for a design.
The retained water affects how liquid is stored and moves through the gel network. Differences in gel water volume can therefore be associated with changes in porosity and permeability, two properties that control transport behavior. Engineers consider these relationships when designing materials for filtration or controlled release, where fluid movement must match the intended function.
Applications that rely on fluid storage or movement can benefit from gel water volume characterization. In filtration systems, it informs properties tied to transport; in controlled release, it relates to fluid retention and movement. The same engineering context supports sensor and tissue-support designs, where swelling and dimensional stability can affect performance.