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A Small Volume Procedure for Viral Concentration from Water
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JoVE Journal Environment
A Small Volume Procedure for Viral Concentration from Water

A Small Volume Procedure for Viral Concentration from Water

DOI:
10.3791/51744-v

•

07:28 min

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February 03, 2015

•

,
1U.S. EPA

Chapters

  • 00:05Title
  • 01:00Preparations
  • 02:30Primary Concentration of Virus from Tap Water
  • 03:23Secondary Concentration Using Celite
  • 06:06Results: Method Optimization
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

An approach was developed for identifying optimal viral concentration conditions for small volume water samples using spikes of human adenovirus. The techniques described here are used to identify concentration parameters for other viral targets, and applied to large-scale viral concentration experimentation.

Tags

Keywords: Virus ConcentrationSmall-volumeWater SampleFiltrationElectropositive FilterElectronegative FilterGlass WoolSize ExclusionSecondary ConcentrationBeef ExtractCeliteOrganic FlocculationElution AdditivesAdenovirusAdV40AdV41

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