In Vitro Suppression Assay
In Vitro Suppression Assay - Related Videos
14:01
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
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2011
Tregs are potent suppressors of the immune system. There is a lack of unique surface markers to define them, hence, definitions of Tregs are primarily functional. Here we describe an optimized in vitro assay capable of identifying immune imbalance in subjects at risk to develop T1D.
02:12
Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing
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2026
Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...
18:48
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
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Cited by 1 •
2017
Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens.
08:34
The Measurement and Treatment of Suppression in Amblyopia
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Cited by 54 •
2012
Amblyopia is a developmental disorder of the visual cortex that is often accompanied by strong suppression of one eye. We present a new technique for measuring and treating interocular suppression in patients with amblyopia that can be deployed using virtual reality goggles or a portable iPod Touch device.
09:55
In vitro Cell Migration and Invasion Assays
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Cited by 627 •
2014
Commonly used, highly accessible methods for examining cell migration and invasion in vitro are described. The first method is the cell wound closure assay that measures cell motility. The second method is the transwell migration and invasion assay that assesses the chemotactic and invasive capacity of cells.
FAQs
Responder T cells may be activated through antigen receptors or by mitogenic signals, and that choice establishes the immune response being tested. Antigen-receptor stimulation examines responses linked to receptor engagement, whereas mitogenic stimulation provides a broader activation context. Comparing suppression under these conditions can help determine whether inhibitory activity depends on the type of responder-cell activation.
The assay can quantify responder-cell proliferation, cytokine production, or activation markers. Proliferation indicates whether cell expansion is reduced, cytokine measurements show changes in functional signaling, and activation markers reveal altered cellular activation. Using one or more of these outcomes allows investigators to distinguish effects on cell growth from effects on immune function or activation state.
A lower responder-cell response in the presence of potential suppressor cells indicates inhibitory activity under the tested culture conditions. The specific outcome can point toward changes in proliferation, cytokine production, or activation. These findings help characterize regulatory mechanisms and can support studies of immune tolerance, where limiting excessive immune activity is biologically important.
First, responder T cells receive an antigen-receptor or mitogenic stimulus. They are then cultured either alone or with a potential suppressor population, such as regulatory T cells, under controlled laboratory conditions. After culture, investigators compare responder-cell proliferation, cytokine production, or activation-marker expression between the conditions to determine the extent of inhibition.
In infection research, the assay can examine how regulatory cellular interactions influence antimicrobial immune responses. Investigators can assess whether pathogen-related conditions alter suppression and thereby affect inflammation, defense, or disease progression. The resulting comparisons provide a controlled way to study how immune regulation shapes cellular responses relevant to infection rather than examining only responder-cell activation alone.
Candidate therapies can be tested by examining whether they alter the suppressive relationship between responder cells and potential suppressor cells. Changes in proliferation, cytokine production, or activation markers indicate how treatment affects cellular immune responses. This makes the assay useful for investigating therapeutic effects on immune regulation, tolerance, and inflammatory activity in a controlled experimental setting.