Metabolic Volume
Metabolic Volume - Related Videos
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
06:59
Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test
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Cited by 7 •
2016
The goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primates and assess their metabolic status from healthy to dysmetabolic.
02:41
Constant Volume Calorimetry
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2020
Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
FAQs
The selected uptake threshold determines which regions are counted as metabolically active, so it directly influences the calculated volume. A defined threshold helps apply the same segmentation rule across scans or study groups, while inconsistent thresholds can make disease-burden comparisons difficult. In infection research, this choice is therefore important when evaluating changes in inflammatory involvement or treatment response.
Metabolic Volume describes the spatial extent of activity, whereas uptake intensity indicates how strongly the tracer accumulates in the measured region. Considering both values can distinguish a smaller, highly active focus from more extensive tissue involvement with different intensity. This combined assessment provides a broader description of disease burden and host responses than either measurement alone.
The distribution of active regions can show whether inflammatory involvement is confined to a focal site or extends across a larger tissue area. That spatial information complements a single hotspot measurement and supports comparisons of disease distribution between subjects or time points. In immunology and infection studies, it can help characterize the extent of the host response.
A PET image is acquired after administration of a metabolic tracer, commonly 18F-fluorodeoxyglucose. Regions showing tracer accumulation are identified, and a predefined uptake threshold is applied to segment the metabolically active tissue. The three-dimensional segmented region is then quantified as a volume, producing a measure that can be compared across disease assessments or treatment time points.
Repeated measurements can show whether the spatial extent of metabolically active tissue changes during treatment. A decrease may indicate reduced involvement, while an increase may reflect greater distribution of activity, although interpretation depends on the accompanying uptake intensity and study context. This longitudinal use supports objective evaluation of therapeutic outcomes in infection and inflammatory disease research.
Metabolic Volume is useful when researchers need to compare how widely disease-related activity affects tissue rather than relying only on the most intense focus. It can support comparisons among disease states, individuals, or treatment stages by quantifying distribution. Combined with tracer uptake intensity, the measure helps evaluate infection severity and characterize variation in host responses.