Dorsal Approach

The dorsal approach is a surgical access route that reaches anatomical structures from their posterior or dorsal surface, allowing clinicians to expose target tissues while planning around surrounding anatomy. During the procedure, an incision and tissue dissection follow a posterior trajectory, with the path determined by the target’s location, depth, and relationship to nerves, blood vessels, and adjacent structures. Used in orthopedic and other surgical settings, this approach supports visualization and treatment of injuries, lesions, or diseased tissues, while precise anatomical knowledge helps guide access, limit tissue disruption, and promote recovery.

Dorsal Approach - Related Videos

Research

JoVE Journal - Neuroscience

An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

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Cited by 7 •

2016

This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

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Cited by 9 •

2017

This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.

Modeling Sensory Axon Regeneration in a Rat Using Dorsal Root Ganglion Injection and Dorsal Root Crush Injury

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2025

Source: Cheah, M., et al. Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration. J. Vis. Exp. (2017).This video demonstrates a step-by-step procedure for injecting a viral suspension into the dorsal root ganglia (DRGs) and inducing a controlled axonal injury via a dorsal root crush in an anesthetized rat. The protocol involves exposing the DRGs, delivering a growth-promoting viral vector to facilitate axonal regeneration, and severing axonal...

Live Imaging of Dorsal Root Axons after Rhizotomy

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Cited by 6 •

2011

An in vivo imaging protocol to monitor primary sensory axons following dorsal root crush is described. The procedures utilize wide-field fluorescence microscopy and thy1-YFP transgenic mice, and permit repeated imaging of axon regeneration over 4 cm in the PNS and axon interactions with the interface of the CNS.

Establishing In Vitro Models of Dorsal Root Ganglia Culture: Complementary Approaches for Investigating Cancer-Nerve Crosstalk

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2025

This protocol describes methods for establishing whole mount and dissociated cultures of mouse Dorsal Root Ganglia (DRG) and their use to assess tumor-nerve interactions.

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