Dual-probe Detection
Dual-probe Detection - Related Videos
11:21
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
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Cited by 5 •
2010
This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.
06:58
Synthesis of an In vivo MRI-detectable Apoptosis Probe
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Cited by 3 •
2012
Early detection of apoptosis may identify at-risk cell populations in a variety of diseases. Here we demonstrate a method to link an early apoptosis-detection protein (Annexin V) to a MRI-detectable iron oxide nanoparticle (SPIO). This method may be extended to other proteins of interest to generate MRI-detectable molecular imaging probes.
02:40
Detecting SMAD Pathway Activation Using Dual Adenovirus Transduction
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2026
Source: Chen, H., et al. Live Cell Imaging of the TGF-β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System. J. Vis. Exp. (2018).This video demonstrates a method to study SMAD pathway activation in breast cancer cells using dual adenoviral infection. One virus delivers a constitutively expressed GFP gene, while the other carries an RFP reporter under a SMAD-sensitive promoter. Upon TGF-beta stimulation, SMAD proteins are activated, forming a complex that induces RFP...
12:52
Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
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Cited by 4 •
2015
Robust detection reagents are of increasing necessity for developing new malaria diagnostic tools. An iridium(III) probe was designed that emits long-lasting luminescent signal in the presence of a histidine-rich malarial protein biomarker. Detection of the protein either in solution or immobilized on a magnetic particle affords flexibility in application.
11:15
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
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Cited by 16 •
2015
Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
FAQs
Confidence increases because the two probes must recognize separate sites on the same biological target before their combined binding produces a measurable readout. This two-site requirement helps distinguish a genuine target-associated signal from an interaction produced by only one probe. The approach is therefore useful when specificity matters in biomarker, pathogen, genetic-sequence, or disease-associated-cell testing.
Binding separate sites provides complementary evidence that the intended molecule, cell, or tissue is present. If both probes associate with their respective sites and generate a combined signal, the result supports target identity more strongly than an isolated interaction would. This arrangement is especially relevant when nonspecific interactions could otherwise complicate interpretation of a diagnostic measurement.
Successful pairing can be represented by fluorescence, luminescence, or another assay readout, depending on the detection system. The important feature is that the measurable output reflects combined probe binding rather than an unconfirmed single interaction. Selecting an appropriate readout allows the same underlying strategy to support different laboratory testing and molecular diagnostic formats.
A general workflow begins by selecting two complementary probes directed at separate sites on the biological target. The probes are then applied to the relevant molecule, cell, or tissue, and the assay is examined for a combined measurable signal. Interpreting that signal in relation to probe pairing helps determine whether the target-associated result is supported.
Medical applications can include detecting biomarkers, identifying pathogens, examining genetic sequences, and characterizing disease-associated cells. Researchers may also use the strategy to study disease mechanisms or treatment response. Its value is greatest when testing requires stronger target specificity or confirmation that an observed signal reflects the biological feature under investigation.
Beyond indicating that a target may be present, paired-probe results can support characterization of the target by confirming recognition at two sites. In medical research, this can strengthen interpretation of findings related to disease-associated cells, biomarkers, pathogens, or genetic sequences. The same confirmation principle can also contribute to studies of disease mechanisms and treatment response.