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Biology

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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
 

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Article DOI: 10.3791/1995
June 26th, 2010

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Summary June 26th, 2010

This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.

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