A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Summary June 26th, 2010
This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.