A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:03Title
- 00:57Introduction
- 01:26Preparing Fluorescent Protein Droplet Samples
- 03:59Setting up a Photoconversion Experiment
- 06:57Dual-Probe Optical Highlighting
- 09:09Representative Results
- 10:48Conclusion
This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.
Tags
PhotoconversionFluorescent ProteinsOptical HighlighterLive CellsMarkingSubpopulationTrackingIntensityExposure TimePhotoconversion LightPhotobleachConfocal Laser Scanning MicroscopePurified Protein Droplet SamplesPhotophysical BehaviorMicroscope ConfigurationDual-probe Optical HighlightingMOrange2Dronpa
