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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
JoVE Journal
Biology
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JoVE Journal Biology
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

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11:21 min

June 26, 2010

DOI:

11:21 min
June 26, 2010

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This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.

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