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June 26, 2010
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This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.
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Cite this Article
Kremers, G., Piston, D. Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells. J. Vis. Exp. (40), e1995, doi:10.3791/1995 (2010).
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