Heart Lung Machine
Heart Lung Machine - Related Videos
06:16
Rat Heterotopic Abdominal Heart/Single-lung Transplantation in a Volume-loaded Configuration
0 Views •
2015
We describe a novel technique for heterotopic abdominal heart-lung transplantation (HAHLT) in rats. The transplant configuration results in a partially loaded graft circulation, allowing direct functional assessment. This model may be employed for acute or chronic studies of function and immunologic status of the transplanted graft.
05:17
Cardiopulmonary Complex Decellularization: A Technique for Decellularizing Heart and Lungs from Murine Model
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2025
This video demonstrates a method of producing decellularized extracellular matrix or ECM scaffolds by applying antegrade perfusion of detergents through the vasculature. The structural ECM scaffolds can then be used to analyze ECM remodeling associated with cardiopulmonary disease.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
08:12
Intramyocardial Cell Delivery: Observations in Murine Hearts
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Cited by 9 •
2014
Intramyocardial cell delivery in murine models of cardiovascular diseases, such as hypertension or myocardial infarction, is widely used to test the therapeutic potential of different cell types in regenerative studies. Therefore, a detailed description and a clear visualization of this surgical procedure will help to define the limits and advantages of cardiovascular cell therapeutic analyses in small rodents.
07:47
A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
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Cited by 12 •
2013
Developmental studies in the mouse are hampered by the inaccessibility of the embryo during gestation. To promote the long-term culture of the embryonic heart at late stages of gestation, we developed a protocol in which the excised heart is cultured in a semi-solid, dilute Matrigel.
FAQs
The reservoir receives venous blood before it is propelled onward, while the oxygenator performs the gas-exchange role normally carried out by the lungs. Together, these components support a controlled circuit that can add oxygen and remove carbon dioxide before blood returns to arterial circulation. Their coordinated operation helps maintain tissue perfusion during procedures requiring the heart to remain still.
Heat exchangers help regulate body temperature while the heart-lung machine supports circulation. Temperature control is important because bypass must maintain physiological support as surgeons work on the heart. In this setting, temperature regulation is part of broader organ-protection research, which examines how conditions during machine-supported circulation may affect tissues and organs.
By diverting blood flow away from the heart and maintaining arterial circulation through the machine, cardiopulmonary bypass allows surgeons to operate while blood flow through the heart is stopped. This produces a relatively still operative field without abandoning tissue perfusion. The combination is particularly valuable when repairing or replacing valves or performing coronary artery bypass surgery.
During bypass, venous blood is directed into a reservoir, then propelled through an oxygenator. The oxygenator adds oxygen and removes carbon dioxide before the blood is returned to the arterial circulation. Heat exchangers can regulate temperature within this support process. This sequence temporarily maintains circulation and gas exchange while the surgical team works on the heart.
The machine supports many open-heart procedures in which surgeons need a relatively still heart and continued tissue perfusion. The overview specifically identifies valve repair, valve replacement, and coronary artery bypass surgery as important examples. Its role is therefore not limited to one operation, but applies across procedures involving structural heart repair or restoration of coronary blood flow.
Use of the system informs research beyond the immediate operation. Scientists study blood compatibility, meaning how circulating blood interacts with the machine, as well as inflammation and organ protection during bypass. These areas help frame the medical consequences of machine-supported circulation and guide investigation of how perfusion conditions may influence tissues and organs.