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May 24, 2013
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Developmental studies in the mouse are hampered by the inaccessibility of the embryo during gestation. To promote the long-term culture of the embryonic heart at late stages of gestation, we developed a protocol in which the excised heart is cultured in a semi-solid, dilute Matrigel.
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Dyer, L. A., Patterson, C. A Novel Ex vivo Culture Method for the Embryonic Mouse Heart. J. Vis. Exp. (75), e50359, doi:10.3791/50359 (2013).
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