Mcao Reperfusion
Mcao Reperfusion - Related Videos
10:30
Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice
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Cited by 16 •
2016
In order to understand the pathophysiology of stroke, it is important to use reliable models. This paper will describe one of the most frequently used stroke models in mice, termed the middle cerebral artery occlusion (MCAo) model (also termed the intraluminal filament or suture model) with reperfusion.
09:40
Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining
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Cited by 97 •
2012
The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.
13:01
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
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Cited by 76 •
2011
The intraluminal middle cerebral artery occlusion (MCAO) model is the most frequent used model among experimental ischemic stroke models. Here we will demonstrate the entire model in detail with the guide of Laser Doppler flowmetry, and its representative results.
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
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Cited by 52 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
06:38
The Stroke Preclinical Assessment Network Multi-Laboratory Model of Thromboembolic Stroke with Thrombolysis: TE-MCAo
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2025
To simulate thromboembolic stroke, emboli prepared from heterologous rat blood were injected into the middle cerebral artery, followed by administration of systemic thrombolysis for recanalization. This version of the model is optimized for use in a multi-laboratory network and supports testing multiple candidate therapeutics.
FAQs
Restoring blood flow does not simply end the experimental injury; it creates a reperfusion phase in which tissue damage can continue. The model therefore separates effects associated with temporary ischemia from later ischemia-reperfusion injury. This distinction helps investigators examine brain damage that appears or worsens after circulation returns, rather than measuring ischemia alone.
The occlusion period provides a defined experimental condition: it determines how long the middle cerebral artery remains blocked before reperfusion begins. Researchers can then examine how that controlled ischemic interval relates to neurological deficits, infarct formation, edema, and inflammatory responses. Keeping the period defined also supports comparisons among experiments evaluating potential neuroprotective treatments.
Neurological deficits provide a functional readout, whereas infarct formation indicates tissue injury. Edema reflects swelling, and inflammatory responses reveal another component of the tissue reaction. Considering these outcomes together gives a broader picture than any single measure: investigators can evaluate functional consequences, structural injury, swelling, and inflammation after the ischemic and reperfusion phases.
The workflow begins by placing an intraluminal filament to obstruct the middle cerebral artery. The obstruction remains for a defined interval, after which the filament is removed to restore blood flow. Researchers then assess the animal for neurological deficits and examine outcomes such as infarct formation, edema, and inflammatory responses. This sequence preserves separate ischemic and reperfusion phases for study.
An intraluminal filament permits the arterial blockage to be reversed within the same experiment. Its removal creates the planned transition from ischemia to reperfusion, allowing investigators to study injury associated with restored circulation. That reversible design makes the procedure suitable for testing whether an intervention limits damage during or after blood flow returns.
Mcao Reperfusion is useful when researchers need an animal model that captures both temporary cerebral ischemia and injury after blood flow is restored. In medicine-focused studies, it supports investigations of stroke mechanisms, evaluation of neuroprotective treatments, and optimization of interventions intended to limit brain damage. Outcomes can be compared through neurological, tissue, swelling, and inflammatory measures.