Micro Slice Viability
Micro Slice Viability - Related Videos
11:13
Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
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Cited by 8 •
2015
Fat grafting is an essential technique for reconstructing soft tissue deficits. However, it remains an unpredictable procedure characterized by variable graft survival. Our goal was to devise a mouse model that utilizes a novel imaging method to compare volume retention between differing techniques of fat graft preparation and delivery.
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
12:03
Viability Assays for Cells in Culture
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Cited by 39 •
2014
Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
08:13
In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography
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Cited by 17 •
2016
This method outlines the use of Quantum Micro-Computed Tomography (MicroCT) to assess cardiac morphology, function, perfusion, metabolism and viability with iodinated contrast agent in mice with experimentally-induced myocardial ischemia. The technique can be applied for non-destructive high-throughput longitudinal in vivo imaging of various animal models of human heart disease.
07:28
Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
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Cited by 6 •
2016
Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.
FAQs
Slice thickness affects how effectively oxygen and nutrients reach cells after tissue architecture has been disrupted. Sections that are not appropriately thin may experience reduced delivery to internal regions, increasing cellular stress and compromising function. For this reason, thickness is evaluated alongside culture conditions when researchers determine whether an ex vivo sample remains suitable for medical experiments.
Viability depends not only on individual cell survival but also on the preservation of local tissue interactions. Slicing can alter the spatial relationships that support tissue-specific behavior, making functional integrity more difficult to maintain outside the body. Preserving these interactions helps researchers study tissue biology and interpret experimental responses in a setting that retains relevant local features.
Researchers assess several complementary indicators rather than relying on a single measurement. Cellular indicators reveal whether cells remain alive, metabolic indicators reflect ongoing biological activity, and structural indicators show whether tissue organization is retained. Considering these categories together helps distinguish preserved function from samples that appear intact but have suffered cellular or metabolic damage.
Preparation requires attention to the consequences of slicing, especially disruption of tissue architecture and restricted oxygen or nutrient delivery. Researchers then maintain the sections under suitable culture conditions and evaluate cellular, metabolic, and structural status. This workflow helps identify preparation-related damage before experimental results are attributed to a drug, disease process, or other treatment.
Viable tissue slices support several medical research applications, including disease modeling, drug response testing, and investigations of tissue-specific biology. Their value comes from retaining living cells and local interactions while allowing experiments outside the body. Assessing sample condition is essential because preparation-related injury could otherwise be mistaken for a disease feature or a treatment effect.
Viability assessment provides a baseline for judging whether observed changes reflect the intervention or damage introduced during sample preparation. Cellular, metabolic, and structural findings can be considered together to establish whether the slice remains experimentally suitable. This distinction is particularly important in drug response studies, where compromised tissue could produce misleading evidence of toxicity or altered biology.