Murine Lung Transplant
Murine Lung Transplant - Related Videos
10:01
Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
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Cited by 20 •
2012
Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.
08:22
Orthotopic Left Lung Transplantation in Rats
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Cited by 1 •
2025
This protocol describes an improved technique for orthotopic left lung transplantation in rats, focusing on reducing operational difficulty and improving survival rates.
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
10:27
Murine Heterotopic Heart Transplant Technique
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Cited by 19 •
2014
The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
FAQs
Varying donor and recipient genetics lets investigators examine how genetic differences shape graft function and immune responses. This controlled comparison helps separate effects associated with the transplanted tissue from those arising in the recipient. In medicine research, such designs are useful for studying why acute rejection develops or why tolerance may emerge under particular experimental conditions.
Researchers can use the model to examine these processes as separate experimental targets by changing relevant conditions. Ischemia-reperfusion injury is studied in relation to the transplant process, acute rejection reflects a graft response associated with immune activity, and tolerance represents a contrasting outcome. Immunosuppressive treatment and experimental injury conditions help create comparisons among these responses.
Immunosuppressive treatment functions as an adjustable experimental variable rather than a fixed feature of the model. Researchers can compare different treatment conditions while monitoring graft function, immune responses, or injury. This design helps investigate how treatment relates to acute rejection and tolerance, and supports evaluation of therapies with potential relevance to human lung transplantation.
The brief preservation interval makes handling and implantation part of a controlled transplant sequence, while also allowing investigators to study injury associated with the transplant process. Because ischemia-reperfusion injury is an explicit research target, the interval can be considered alongside post-implantation graft function and tissue findings.
Longitudinal analysis follows changes over time rather than relying only on a single tissue sample. In murine lung transplant studies, researchers can pair this approach with controlled tissue sampling to examine evolving graft function, immune responses, and transplant injury. The resulting time-resolved view can clarify how experimental conditions influence rejection, tolerance, or other outcomes.
It serves as a controlled platform for transplant biology, not merely as a demonstration of surgical reconnection. Investigators can alter genetics, immunosuppressive treatment, or injury conditions, then assess graft-related outcomes through tissue sampling and longitudinal analysis. These capabilities support mechanistic studies and the evaluation of therapies relevant to human lung transplantation.