Dapi Alexa Fluor 555
Dapi Alexa Fluor 555 - Related Videos
09:11
Visualizing Dengue Virus through Alexa Fluor Labeling
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Cited by 12 •
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Taking advantage of the advancements in fluorophore development and imaging technology, a simple method of Alexa Fluor labeling of dengue virus was devised to visualize the early interactions between virus and cell.
03:32
Ethanol Fixation and DAPI Staining: A Method to Visualize DNA in C. elegans
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Here, we describe a technique used to visualize genomic DNA in fixed, intact nematodes. The video includes an example protocol to count chromatin in unfertilized C. elegans oocytes.
03:23
Cell Death Screening Using DAPI Staining: A Method for Rapid Screening of IR Induced Clonogenic Cell Death in Cancerous Cell Lines
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This video demonstrates the methodology to screen for major cell death profiles in ionizing radiation (IR) induced cancer cell lines using DAPI staining assay. The DAPI-stained nuclei of target cells undergoing cell death exhibit distinct morphologies and can be easily identified by fluorescence microscopy.
03:16
Three-dimensional Imaging of Immunolabeled Astrocytes Using Confocal Microscopy
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2025
Source: Bagheri, M., et. al. Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes. J. Vis. Exp. (2015).This video demonstrates the imaging of fluorescently labeled astrocytes in a mouse brain section using a confocal microscope. The brain section is positioned under the appropriate objective lens, and suitable fluorophores and filters are selected. After optimizing the exposure automatically, the scanning parameters are defined to ensure high-resolution 2D images. Z-stack...
06:04
C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
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Cited by 7 •
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This is a commonly used method for C. elegans gonad dissection followed by freeze crack, which produces germline samples for immunofluorescence via antibody staining, or for simple DAPI staining to visualize DNA. This protocol has been successful for undergraduates in a research lab and in a course-based undergraduate research experience.
FAQs
The two labels produce spectrally distinct fluorescence signals: DAPI appears blue when appropriately excited, whereas Alexa Fluor 555 produces orange-red emission. Multichannel fluorescence microscopy records these signals separately, allowing nuclear features and the labeled molecular target to be viewed within one fixed sample. This separation supports direct comparison of cellular location and tissue organization.
Alexa Fluor 555 does not identify a structure by itself; its attached antibody or other probe determines the target. The probe recognizes a selected antigen, such as a neuronal protein, glial marker, or synaptic component, while DAPI provides the nuclear reference. Changing the probe therefore changes the molecular feature examined without changing the nuclear labeling channel.
Nuclear fluorescence provides a positional framework for interpreting the orange-red target signal. Investigators can relate labeled molecular features to individual nuclei, surrounding cells, or regions within brain tissue and cultured neural cells. This paired spatial information makes it possible to assess where a target occurs and how its distribution relates to cellular organization.
A typical workflow uses a fixed biological sample, applies DAPI to label DNA, and uses an antibody or other Alexa Fluor 555-linked probe to recognize the selected antigen. The specimen is then examined with multichannel fluorescence microscopy, with blue and orange-red signals evaluated separately or together to interpret nuclear and molecular-target patterns.
The Alexa Fluor 555 channel can be directed toward neuronal proteins, glial markers, synaptic components, or other selected antigens, depending on the probe used. DAPI supplies nuclear context for each observation. Consequently, the same imaging strategy can support studies of brain tissue architecture as well as molecular organization in cultured neural cells.
Images generated with this combination can support cell counting, anatomical localization, colocalization analysis, and evaluation of cellular organization. Nuclear signal helps identify and spatially reference cells, while the Alexa Fluor 555 signal maps the selected target. In neuroscience, these outcomes help describe how molecular features are distributed across brain tissue or neural cultures.