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C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining
JoVE Journal
Genetics
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JoVE Journal Genetics
C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

C. elegans Gonad Dissection and Freeze Crack for Immunofluorescence and DAPI Staining

DOI:
10.3791/64204-v

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06:04 min

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September 16, 2022

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, , ,
1Department of Biological Sciences,University of Massachusetts Lowell

Chapters

  • 00:05Introduction
  • 00:43Gonad Dissection
  • 04:23Results: Visualizing Meiotic Chromosomes in C. elegans
  • 05:10Conclusion

Summary

Automatic Translation

Automatic Translation

This is a commonly used method for C. elegans gonad dissection followed by freeze crack, which produces germline samples for immunofluorescence via antibody staining, or for simple DAPI staining to visualize DNA. This protocol has been successful for undergraduates in a research lab and in a course-based undergraduate research experience.

Tags

Keywords: C. ElegansGonad DissectionImmunofluorescenceDAPI StainingFreeze CrackDissection ProtocolGermline ExtrusionFormaldehyde FixationLiquid NitrogenPositively-charged Slide

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