Fluorescence Plate Scanner
Fluorescence Plate Scanner - Related Videos
08:25
Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
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Cited by 6 •
2014
This protocol describes a process for rapid collection of images of Arabidopsis seedlings responding to a gravity stimulus using commercially-available flatbed scanners. The method allows for inexpensive, high-volume capture of high-resolution images amenable for downstream analysis algorithms.
07:01
Frequency Mixing Magnetic Detection Scanner for Imaging Magnetic Particles in Planar Samples
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Cited by 5 •
2016
A scanner for imaging magnetic particles in planar samples was developed using the planar frequency mixing magnetic detection technique. The magnetic intermodulation product response from the nonlinear nonhysteretic magnetization of the particles is recorded upon a two-frequency excitation. It can be used to take 2D images of thin biological samples.
13:29
Jet Impinging on an Inclined Plate
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The goal of this experiment is to demonstrate how a fluid flow exerts forces on structures by conversion of dynamic pressure into static pressure. To this end, we will make a plane jet impinge on a flat plate and will measure the resulting pressure distribution along the plate. The resultant force will be estimated by integrating the product between the...
07:04
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
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Cited by 2 •
2014
Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.
10:53
Serial Dilutions and Plating: Microbial Enumeration
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2023
Source: Jonathan F. Blaize1, Elizabeth Suter1, and Christopher P. Corbo1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Quantitative assessment of prokaryotes can be onerous given their abundance, propensity for exponential proliferation, species diversity within a population, and specific physiological needs. Compounding this challenge, is the four-phase nature in which bacteria replicate (lag, log, stationary and death). The ability to accurately...
FAQs
The instrument directs selected excitation wavelengths into each well, then collects the resulting emitted light through optical filters or monochromators. It converts the detected fluorescence into signal-intensity data, allowing measurements from multiple wells to be compared. Because the same process can be applied across several wavelengths or time points, researchers can examine changes in fluorescent readouts systematically.
Excitation wavelengths determine which fluorescent signals are stimulated in the sample, while filters or monochromators help select the emitted light reaching the detector. This separation connects the measured signal to the intended fluorescent readout. Using selected optical settings also enables collection across multiple wavelengths, supporting more detailed analysis than a single fluorescence measurement.
Measurements at multiple wavelengths can distinguish or compare different fluorescence-based readouts, whereas repeated measurements over time reveal how signal intensity changes. This is especially useful when a biological reaction or cellular state develops dynamically rather than remaining constant. The resulting data can support quantitative comparisons among samples, conditions, or stages of an experiment.
Microplate wells organize many samples so that the scanner can analyze them in parallel under selected optical conditions. This arrangement supports rapid comparison of biological reactions or cellular states across numerous samples. In practice, parallel measurement helps researchers evaluate fluorescence intensity across experimental conditions and identify differences in signaling, activity, or reporter output.
Researchers arrange neuronal cultures or tissue-derived samples in microplate wells, choose excitation and emission settings appropriate to the fluorescent readout, and scan the plate. They may collect measurements across multiple wavelengths or time points, then compare signal intensities among wells. This workflow links fluorescence changes to cellular states, biological reactions, or experimentally tested conditions.
In neuronal cultures or tissue-derived samples, fluorescence measurements can serve as readouts of intracellular calcium dynamics. Researchers track signal intensity across wells or time points to compare calcium-related changes among experimental conditions. These data help investigate neuronal signaling and can contribute to studies of how cellular responses vary during pharmacological experiments or disease-related investigations.
The approach can measure fluorescence-based indicators of receptor activity, enzyme activity, and gene-expression reporters in neuronal cultures or tissue-derived samples. Each readout connects detected signal intensity with a different cellular process. Consequently, plate-based measurements support broader investigations of neuronal signaling, pharmacology, and disease mechanisms without restricting analysis to a single type of biological response.