Miller Reedy Protocol
Miller Reedy Protocol - Related Videos
11:10
Conducting Miller-Urey Experiments
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Cited by 16 •
2014
The Miller-Urey experiment was a pioneering study regarding the abiotic synthesis of organic compounds with possible relevance to the origins of life. Simple gases were introduced into a glass apparatus and subjected to an electric discharge, simulating the effects of lightning in the primordial Earth’s atmosphere-ocean system. The experiment was conducted for one week, after which, the samples collected from it were analyzed for the chemical building blocks of life.
10:50
Denver Papillae Protocol for Objective Analysis of Fungiform Papillae
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Cited by 47 •
2015
Here we present a protocol to measure fungiform papilla density from digital photographs. This method builds prioritization and objective characteristic metrics into the original descriptive work on fungiform papillae by Miller & Reedy (1990).
12:12
Echocardiographic Approaches and Protocols for Comprehensive Phenotypic Characterization of Valvular Heart Disease in Mice
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Cited by 15 •
2017
This protocol provides a detailed description of the echocardiographic approach for comprehensive phenotyping of heart and heart valve function in mice.
10:25
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
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Cited by 15 •
2011
The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.
07:42
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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Cited by 8 •
2010
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
FAQs
Selective silver impregnation makes neuronal features visible by causing staining reagents to deposit along cellular structures within fixed nervous tissue. Because the deposition follows structures rather than uniformly coloring the surrounding material, somata, dendrites, and axons can be examined as separate morphological elements. This mechanism supports microscopic assessment of neuronal form and structural relationships.
Fixation and processing consistency directly affect whether the protocol produces interpretable labeling. The tissue must undergo controlled processing, while reagent handling and exposure times remain consistent across samples. Variations in these conditions can alter the resulting impregnation and complicate comparisons between specimens, making standardized treatment important for reliable morphological analysis.
After labeling, sectioning and light microscopy allow investigators to examine the arrangement of visible neuronal components in tissue context. They can distinguish somata, dendrites, and axons and use those observations to assess morphology and connectivity. Careful interpretation remains necessary because conclusions depend on correctly identifying labeled structures rather than treating every deposit as equivalent.
A typical workflow begins with consistent fixation of nervous tissue, continues through controlled processing and selective silver impregnation, and then proceeds to sectioning. The prepared sections are examined with a light microscope, where labeled neuronal structures are assessed. Maintaining reagent handling and processing times throughout the workflow helps produce results suitable for comparison.
The protocol can provide information about neuronal morphology, including the appearance and organization of somata, dendrites, and axons. Examination of labeled sections also supports assessment of connectivity and structural changes in experimental models. These observations allow researchers to relate microscopic neuronal organization to broader questions about nervous-tissue structure and organization.
It is useful when researchers need to examine neuronal structure in studies of brain organization, development, injury, or disease. By making cellular features visible for microscopic analysis, the method supports comparisons of neuronal morphology, connectivity, and structural changes across experimental models. Its value depends on consistent processing and careful interpretation of the labeled tissue.