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JoVE Journal
Chemistry
Conducting Miller-Urey Experiments
Conducting Miller-Urey Experiments
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Conducting Miller-Urey Experiments

Conducting Miller-Urey Experiments

Full Text
70,743 Views
11:10 min
January 21, 2014

DOI: 10.3791/51039-v

Eric T. Parker1, James H. Cleaves2,3, Aaron S. Burton4, Daniel P. Glavin5, Jason P. Dworkin5, Manshui Zhou1, Jeffrey L. Bada6, Facundo M. Fernández1

1School of Chemistry and Biochemistry,Georgia Institute of Technology, 2Earth-Life Science Institute,Tokyo Institute of Technology, 3Institute for Advanced Study, 4Astromaterials Research and Exploration Science Directorate,NASA Johnson Space Center, 5Goddard Center for Astrobiology,NASA Goddard Space Flight Center, 6Geosciences Research Division, Scripps Institution of Oceanography,University of California at San Diego

The Miller-Urey experiment was a pioneering study regarding the abiotic synthesis of organic compounds with possible relevance to the origins of life. Simple gases were introduced into a glass apparatus and subjected to an electric discharge, simulating the effects of lightning in the primordial Earth’s atmosphere-ocean system. The experiment was conducted for one week, after which, the samples collected from it were analyzed for the chemical building blocks of life.

The overall goal of the following experiment is to conduct a Miller Yuri Spark discharge experiment. This is achieved by placing water into a reaction flask to represent the early earth's oceans. As a second step, a suite of gases is introduced into the reaction flask to mimic primitive atmospheric conditions.

Next, an electric discharge is applied within the reaction flask. In order to imitate lightning on the primordial earth, results are obtained that show molecules important for life can be detected based on high performance liquid chromatography with fluorescence detection, amino acid analysis of spark discharge samples, The Miller U Experiment can help answer key questions in the origins of life field, such as which types of prebiotic conditions may have facilitated the synthesis of specific organic compounds believed to be important for life. To begin this procedure, set up the manifold and vacuum system as described in the text protocol.

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