Mouse Cortical Tissue
Mouse Cortical Tissue - Related Videos
10:50
Isolation of Pericytes from Mouse Cortical Tissue Using FACS for Single-cell Sequencing
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Cited by 1 •
2026
To obtain mouse brain cortical pericytes meeting the demands of scRNA-seq, we present a critically optimized FACS protocol based on CD13⁺/CD31⁻ selection. Our key refinements significantly enhance cell viability and RNA integrity, enabling reliable transcriptomic profiling.
11:25
Isolation and Culture of Mouse Cortical Astrocytes
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Cited by 460 •
2013
Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.
18:02
Preparation of Dissociated Mouse Cortical Neuron Cultures
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Cited by 146 •
2007
This video shows a procedure for generating neuronal cultures from late embryo and early postnatal mouse cortex. These cultures can be used for immunocytochemistry, biochemistry, electrophysiology, calcium and sodium imaging and provide a platform to study the neuronal development of transgenic animals that carry a postnatal lethal gene mutation.
06:41
Astrocyte Isolation: A Method to Obtain Pure Preparation of Mouse Cortical Astrocytes
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2023
In this video, we describe the method to obtain a pure preparation of astrocytes from newly born mice.
15:40
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
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Cited by 63 •
2011
This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.
FAQs
Cortical function emerges from interactions among neurons and glial cells rather than from isolated cells acting independently. Neuronal electrical activity travels along membranes, while local circuits organize how signals are distributed across connected cells. Ion channels influence membrane activity, and glial regulation modifies the surrounding neural environment, together shaping sensory processing, cognition, and coordinated behavior.
Information transfer begins when electrical activity moves along a neuronal membrane and reaches a synapse. This activity triggers neurotransmitter release, allowing one neuron to influence another. Ion channels then help determine how the receiving cell responds, while local circuit organization shapes the broader pattern of activity. Studying this sequence reveals how cellular events contribute to cortical function.
Ion channels help control the electrical behavior of neuronal membranes, whereas local circuits determine how activity spreads and interacts within cortical networks. Examining both levels is important because a change in membrane signaling may produce different outcomes depending on circuit connections. Mouse cortical tissue therefore supports investigations that link cellular electrical mechanisms with network-level neural responses.
Glial cells contribute to neural function by regulating the environment in which neurons communicate. Their influence can modify how synaptic signaling operates alongside electrical activity, neurotransmitter release, and ion-channel function. Including glial regulation in cortical studies helps researchers interpret neuronal behavior within a tissue context rather than treating synapses as independent communication sites.
Three commonly described formats are freshly isolated cortex, organotypic slices, and primary cortical cultures. These preparations provide different ways to examine cortical biology while preserving different amounts of tissue organization or cellular accessibility. The appropriate format depends on whether a study emphasizes intact local relationships, isolated cellular responses, development, synaptic plasticity, neurodegeneration, or responses to drugs or injury.
Mouse cortical preparations support studies of development, neurophysiology, synaptic plasticity, neurodegeneration, and responses to drugs or injury. Researchers can use these models to examine how neuronal signaling and local tissue interactions change under different biological or experimental conditions. Their value comes from making cellular mechanisms accessible while retaining a connection to processes relevant to brain function.
These preparations help connect cellular mechanisms with broader changes in brain function and disease. Investigators can examine synaptic communication, electrical activity, local circuits, and glial regulation in relation to neurodegeneration or injury responses. Drug studies likewise use cortical models to assess how neural tissue responds, providing a controlled bridge between cellular observations and disease-relevant neuroscience questions.