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Isolation and Culture of Mouse Cortical Astrocytes
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Mouse Cortical Astrocytes

Isolation and Culture of Mouse Cortical Astrocytes

DOI:
10.3791/50079-v

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11:25 min

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January 19, 2013

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, , ,
1Institute of Anatomy and Cell Biology,University of Freiburg , 2Centre of Chronic Immunodeficiency (CCI), University Medical Centre Freiburg,University of Freiburg

Chapters

  • 00:05Title
  • 02:32Dissection of Cortical Tissue
  • 04:51Plating Mixed Cortical Cells
  • 06:38Obtaining an Enriched Astrocyte Culture
  • 09:11Results: Morphology and Purity of Astrocyte Cultures
  • 10:53Conclusion

Summary

Automatic Translation

Automatic Translation

Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.

Tags

Keywords: Mouse Cortical AstrocytesAstrocyte Cell CultureIn Vitro Astrocyte CultureGlial Cell SeparationAstrocyte MarkersAstrocyte MorphologyAstrocyte-conditioned Medium

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