Neonatal Animals
Neonatal Animals - Related Videos
07:54
Isolation of Neonatal Extrahepatic Cholangiocytes
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Cited by 17 •
2014
A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
10:36
A Swine Model of Neonatal Asphyxia
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Cited by 50 •
2011
Large animal models have good translational values in the examination of physiology and pharmacology of neonatal asphyxia. Using newborn piglets, we develop an experimental protocol to simulate neonatal asphyxia which has advantages of studying the systemic and regional hemodynamics, oxygen transport with biochemical and pathologic pathways and correlations.
10:30
A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy
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Cited by 37 •
2015
Hypoxic-ischemic encephalopathy following perinatal asphyxia can be studied using animal models. We demonstrate the procedures necessary for establishing a piglet model of neonatal hypoxic-ischemic encephalopathy.
06:27
Transcutaneous Microcirculatory Imaging in Preterm Neonates
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Cited by 6 •
2015
Microcirculatory imaging (MI) is used to monitor peripheral perfusion in critically ill or preterm neonates. This manuscript and video demonstrates the optimal approach for obtaining high-quality images.
FAQs
The early postnatal nervous-system changes make age a central experimental variable. Neural circuits are forming and refining during this period, so results from animals at different ages may reflect different stages of maturation rather than the same biological response. Recording age precisely helps researchers relate brain function to a defined developmental stage.
Sensory-driven activity provides functional input while synaptic growth changes the connections through which neurons communicate. Together, these processes influence how immature circuits are assembled and refined. Examining them helps distinguish changes associated with circuit formation from changes that reflect later neural organization, making early activity relevant to studies of sensory processing.
Neuronal excitability, meaning the changing patterns by which neurons respond and generate activity, is a key indicator of circuit maturation. In neonatal animals, its pattern can be examined alongside synaptic growth and sensory-driven activity to understand how functional networks develop. This connection helps researchers interpret whether observed differences reflect altered brain function during early development.
Age, environment, and other experimental conditions require deliberate control because each can influence measurements of brain function. A study can compare animals at defined early postnatal stages while maintaining consistent surroundings and procedures. Controlling these variables makes it easier to attribute differences in neural activity, reflexes, or sensory processing to the factor being investigated.
These studies can provide information about brain function, reflexes, sensory processing, and the relationship between cellular mechanisms and behavior. Researchers can use such outcomes to examine how early neural changes correspond with later behavioral or neurological results. This range of outcomes makes the models useful for connecting events in developing circuits with broader functional consequences.
Neonatal animal models allow researchers to examine brain changes associated with injury and to assess treatments within a period of active nervous-system maturation. Findings can connect cellular mechanisms with brain function and later neurological outcomes. This context is valuable because it places disease-related or treatment-related effects within the developmental trajectory of neural circuits and behavior.