Indirect Coculture Assay
Indirect Coculture Assay - Related Videos
11:08
The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
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Cited by 26 •
2016
This protocol describes the indirect neuron-astrocyte coculture for compartmentalized analysis of neuron-glia interactions.
14:22
ELISA Assays: Indirect, Sandwich, and Competitive
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2023
Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4 1 Department of Urology, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Enzyme-linked immunosorbent assay (ELISA) is frequently used to measure the presence...
09:23
Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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Cited by 19 •
2016
Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
08:23
Comet Assay as an Indirect Measure of Systemic Oxidative Stress
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Cited by 23 •
2015
Comet assay measures DNA breaks, induced by different factors. If all factors (except oxidative stress) causing DNA damage are kept constant, the amount of DNA damage is a good indirect parameter of oxidative stress. The goal of this protocol is to use comet assay for indirect measurement of oxidative stress.
01:30
Indirect Costs
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2026
Indirect costs are costs that cannot be directly traced to a single product, service, or cost object. Instead, they support multiple products or activities simultaneously and must be allocated using a systematic and reasonable method. Cost allocation is the process of distributing these shared costs among cost objects so that each product bears a fair share of the total overhead. Accurate allocation of indirect costs is essential for determining product costs, setting prices, evaluating...
FAQs
The porous membrane creates two essential conditions at once: it separates the cell populations physically while allowing soluble substances to move between compartments. This design focuses interpretation on secreted communication, including cytokines, growth factors, and neurotransmitter-related signals. Because the cells cannot touch or mix, observed changes can be linked more specifically to paracrine influences rather than direct contact.
An Indirect Coculture Assay can be used to examine soluble factors released by one cell population and received by another. Relevant signaling categories include cytokines, growth factors, and neurotransmitter-related signals. In neuroscience, these factors may help explain how glial cells influence neuronal survival, inflammation, differentiation, or synaptic function without requiring the two populations to occupy the same compartment.
The key distinction is whether the cell populations can physically contact one another. Indirect coculture prevents direct contact and cell mixing, so the experiment emphasizes secreted, paracrine communication. A direct coculture would not isolate those signals from contact-dependent mechanisms as clearly. This comparison helps researchers determine whether a neural effect depends primarily on soluble communication or cellular contact.
The two cell populations are maintained in separate chambers or compartments divided by a porous membrane. One population can function as the signaling source, while the other serves as the responding culture. Soluble factors pass between the compartments during coculture, and the recipient population can then be evaluated for changes such as altered survival, inflammation, differentiation, or synaptic function.
This approach is useful when researchers want to study communication between neurons and glial cells while separating secreted effects from direct cellular interactions. It can support investigations of neural development, injury, and disease, particularly when the central question concerns how one population changes another through soluble signals. The same design can examine several response categories, including survival and synaptic function.
Responses in the receiving population can indicate how secreted signals alter neural biology. Depending on the experimental question, measurements may address neuronal survival, inflammatory effects, cellular differentiation, or synaptic function. Interpreting these outcomes in the separated-compartment design helps identify paracrine influence and can clarify how neuron-glia communication contributes to development, injury responses, or disease-related processes.