Isolation of Neonatal Extrahepatic Cholangiocytes

Journal

Biology

JoVE Journal
Biology

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JoVE Journal Biology

Isolation of Neonatal Extrahepatic Cholangiocytes

DOI:

10.3791/51621-v

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07:54 min

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June 05, 2014

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Sara Karjoo, Rebecca G. Wells

¹Division of Gastroenterology, Hepatology, and Nutrition,The Children’s Hospital of Philadelphia, ²Department of Medicine,The Perelman School of Medicine at the University of Pennsylvania

Chapters

00:05Title
01:12Bile Duct Isolation
02:59Embedding Tissues into Thick Collagen Gels
04:19Isolating Primary Cholangiocytes
07:05Characterizing Cultured Cholangiocytes
07:24Conclusion

Summary

Automatic Translation

A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.

Isolation of Neonatal Extrahepatic Cholangiocytes

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