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JoVE Journal
Biology

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Isolation of Neonatal Extrahepatic Cholangiocytes
 

Isolation of Neonatal Extrahepatic Cholangiocytes

Article DOI: 10.3791/51621
June 5th, 2014

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Summary June 5th, 2014

A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.

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