Ftir Analysis
Ftir Analysis - Related Videos
10:06
Characterization Of Multi-layered Fish Scales (Atractosteus spatula) Using Nanoindentation, X-ray CT, FTIR, and SEM
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Cited by 15 •
2014
This paper presents the methods used for probing spatially correlated chemical, structural, and mechanical properties of the multilayered scale of Atractosteus spatula (A. spatula) using nanoindentation, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X-ray computed tomography (X-ray CT). The experimental results have been used to investigate the design principles of protective biological materials.
11:38
In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework
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Cited by 14 •
2020
The use of FTIR spectroscopy for investigation of the surface properties of polycrystalline solids is described. Preparation of sample pellets, activation procedures, characterization with probe molecules and model studies of CO2 adsorption are discussed.
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
10:39
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
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Cited by 3 •
2022
Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
FAQs
The interferometer records an interferogram, which contains the infrared response produced by the sample. A Fourier transform mathematically converts this recorded signal into a spectrum organized by wavenumber. This conversion is central to the analysis because it turns the instrument’s measured signal into a form that can be examined for absorption bands associated with the material.
Molecular bonds absorb particular infrared frequencies when they undergo vibrational motion. These frequency-specific absorptions appear as bands in the spectrum, providing clues about chemical composition and structural features. Interpreting the positions and presence of those bands allows FTIR analysis to distinguish materials and identify chemical changes reflected in their molecular bonding.
Wavenumber provides the spectral coordinate used to locate infrared absorption features. Because molecular bonds respond at characteristic infrared frequencies, the positions of bands along the wavenumber scale help connect measured radiation interactions with particular structural or compositional features. The resulting intensity-versus-wavenumber display is therefore the main basis for interpreting the sample.
The instrument directs a broad infrared beam through the sample or onto its surface so the radiation can interact with the material. The interferometer records the resulting interferogram, and a Fourier transform produces the spectrum. Analysts then examine the intensity pattern and absorption bands to characterize the sample’s composition or structure.
Researchers apply FTIR analysis when they need to identify a material, examine its surface, detect contamination, or monitor chemical changes. The method is relevant to polymers, semiconductors, pharmaceuticals, and environmental samples. Its value comes from connecting measured infrared absorption features with chemical composition and structural information in the investigated material.
In physics, FTIR analysis links the interaction of infrared radiation with a sample to observable structural and compositional features. This makes it useful for studying material identity, surface condition, contamination, and chemical evolution. Applications across polymers, semiconductors, pharmaceuticals, and environmental samples demonstrate its role in examining diverse physical materials and systems.