This content is Free Access.
Chapters
- 00:05Title
- 00:48Introduction
- 01:28Cell Preparation for Neurotoxicity Screens
- 02:57Cell Fixation and Immunofluorescent Staining
- 06:07HCS Imaging, Analysis, and Results
- 14:15Conclusion
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
Tags
Neuronal And Astrocyte Co-culture AssayHigh Content AnalysisNeurotoxicityDrug Safety EvaluationEnvironmental ProtectionNeurodegenerative DiseasesAlzheimer’s DiseaseParkinson’s DiseaseNeuronal ScreeningβIII-tubulin LabelingHigh-throughputQuantitative MeasurementsNeuronal NumberNeurite CountNeurite LengthNeurotoxic EffectsAstrocytes RoleCentral Nervous System HomeostasisNeuroprotectionNeurodegradationGFAP Upregulation
