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Journal / Biology / Hi-C: un metodo per studiare l'architettura…
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Biology

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Hi-C: un metodo per studiare l'architettura tridimensionale dei genomi.

Article DOI: 10.3791/1869-v • 22:27 min • May 6th, 2010
May 6th, 2010 •
*1, *2,3,4,5, *2, 6, 2, 3,6, 1, 2,7,8
1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology, Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University, 5Department of Applied Mathematics, Harvard University, 6Department of Physics, Massachusetts Institute of Technology, 7Department of Systems Biology, Harvard Medical School, 8Department of Biology, Massachusetts Institute of Technology
* These authors contributed equally

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L'Hi-C metodo consente l'identificazione imparziali, genoma delle interazioni della cromatina (1). Hi-C coppie legatura di prossimità e di sequenziamento massicciamente parallelo. I dati ottenuti possono essere usati per studiare architettura genomica alle scale multiple: risultati iniziali identificate caratteristiche come territori cromosoma, la segregazione della cromatina aperti e chiusi, e la struttura della cromatina alla scala megabase.

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Biologia Cellulare Numero 39 la cattura Cromosoma conformazione la struttura della cromatina Illumina la sequenza finale appaiati fisica dei polimeri.
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