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Journal / Biology / DNA-Extraktion aus in Paraffin eingebettete…
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DNA-Extraktion aus in Paraffin eingebettete Material für genetische und epigenetische Analysen
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DNA-Extraktion aus in Paraffin eingebettete Material für genetische und epigenetische Analysen

Article DOI: 10.3791/2763-v • 13:33 min • March 26th, 2011
March 26th, 2011 •
*1,2, *1,2, 3, 1,2,4
1Department of Integrative Oncology, BC Cancer Research Centre, 2Interdisciplinary Oncology Program, University of British Columbia - UBC, 3Photography/Video Production, Multi-Media Services, BC Cancer Agency, 4Department of Pathology and Laboratory Medicine, University of British Columbia - UBC
* These authors contributed equally

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Dieses Video zeigt das Protokoll für die DNA-Extraktion aus Formalin-fixierten Paraffin-eingebetteten Material. Dies ist eine mehrtägige Prozedur, in der Gewebeschnitte werden deparaffinierten mit Xylol, rehydriert mit Ethanol und mit Proteinase K zu reinigen und zu isolieren DNA für die nachfolgende Gen-spezifischen oder genomweite Analyse.

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Genetik DNA-Extraktion in Paraffin eingebetteten Gewebe Phenol: Chloroform-Extraktion genetische Analyse epigenetische Analyse
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